A RhoGAP protein as a main immune suppressive factor in the Leptopilina boulardi (Hymenoptera, Figitidae) - Drosophila melanogaster interaction

被引：96

作者：

Labrosse, C ^{[1
]}

Staslak, K ^{[1
]}

Lesobre, J ^{[1
]}

Grangeia, A ^{[1
]}

Huguet, E ^{[1
]}

Drezen, JM ^{[1
]}

Poirie, M ^{[1
]}

机构：

[1] Univ Tours, Inst Rech Biol Insecte, UPRESA, CNRS 6035,IFR Agents Transmissibles & Infect, F-37200 Toulon, France

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2005年 / 35卷 / 02期

关键词：

Drosophila melanogaster; Leptopilina boulardi; parasitoid wasps; immune suppressive factors; encapsulation; RhoGAP;

D O I：

10.1016/j.ibmb.2004.10.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To protect its eggs. the endoparasitoid wasp Leptopilina boulardi injects immune suppressive factors into Drosophila melanogaster host larvae. These factors are localized in the female long gland and reservoir. We analyzed the protein content of these tissues and found that it strongly differed between virulent and avirulent parasitoid strains. Four protein bands present in virulent long,glands were eluted and their immune suppressive effect was assessed in vivo, allowing demonstrating a major effect of one of these proteins. The corresponding cDNA encodes a predicted 30 kDa Subunit containing a Ras homologous GTPase Activating Protein (RhoGAP) domain, suggesting a possible involvement in the regulation of actin cytoskeleton changes. Using Western-blot experiments, we showed that this protein is abundant ill Virulent female long glands but is undetectable in virulent females deprived of long glands or in long glands from avirulent wasps. Its potential role in modifying the morphology and the adhesive properties of the host lamellocytes involved in Drosophila cellular immune responses, is discussed. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：93 / 103

页数：11

共 41 条

[31] Sequence analysis and expression of a virus-like particle protein,VLP2, from the parasitic wasp Venturia canescens [J].