Single-cell transcriptome conservation in cryopreserved cells and tissues

被引：127

作者：

Guillaumet-Adkins, Amy ^{[1
,2
]}

Rodriguez-Esteban, Gustavo ^{[1
,2
]}

Mereu, Elisabetta ^{[1
,2
]}

Mendez-Lago, Maria ^{[1
,2
]}

Jaitin, Diego A. ^{[3
]}

Villanueva, Alberto ^{[4
,5
]}

Vidal, August ^{[6
]}

Martinez-Marti, Alex ^{[7
,8
,9
]}

Felip, Enriqueta ^{[7
,8
,9
]}

Vivancos, Ana ^{[9
]}

Keren-Shaul, Hadas ^{[3
]}

Heath, Simon ^{[1
,2
]}

Gut, Marta ^{[1
,2
]}

Amit, Ido ^{[3
]}

Gut, Ivo ^{[1
,2
]}

Heyn, Holger ^{[1
,2
]}

机构：

[1] Barcelona Inst Sci & Technol, Ctr Genom Regulat CRG, CNAG CRG, Barcelona, Spain

[2] Univ Pompeu Fabra, Barcelona, Spain

[3] Weizmann Inst Sci, Dept Immunol, Rehovot, Israel

[4] Catalan Inst Oncol ICO, Bellvitge Inst Biomed Res IDIBELL, Program Canc Therapeut Resistance ProCURE, Chemoresistance & Predict Factors Lab, Barcelona, Spain

[5] Xenopat SL, Business Bioincubator, Bellvitge Hlth Sci Campus, Barcelona, Spain

[6] Univ Hosp Bellvitge IDIBELL, Dept Pathol, Barcelona, Spain

[7] Vall dHebron Univ Hosp, Barcelona, Spain

[8] Univ Autonoma Barcelona, Barcelona, Spain

[9] Vall dHebron Inst Oncol VHIO, Barcelona, Spain

来源：

GENOME BIOLOGY | 2017年 / 18卷

关键词：

Single-cell genomics; RNA sequencing; Transcriptomics; MARS-Seq; Smart-seq2; Cryopreservation; Conservation; Peripheral blood mononuclear cells; PBMC; Patient-derived orthotopic xenograft; PDOX; RNA-SEQ; EXPRESSION PROFILES; OVARIAN-CANCER; STEM-CELLS; HETEROGENEITY; DYNAMICS; NEURONS; PATHWAY;

D O I：

10.1186/s13059-017-1171-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

A variety of single-cell RNA preparation procedures have been described. So far, protocols require fresh material, which hinders complex study designs. We describe a sample preservation method that maintains transcripts in viable single cells, allowing one to disconnect time and place of sampling from subsequent processing steps. We sequence single-cell transcriptomes from > 1000 fresh and cryopreserved cells using 3'-end and full-length RNA preparation methods. Our results confirm that the conservation process did not alter transcriptional profiles. This substantially broadens the scope of applications in single-cell transcriptomics and could lead to a paradigm shift in future study designs.

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