Self-Assembled Human Adipose-Derived Stem Cell-Derived Extracellular Vesicle-Functionalized Biotin-Doped Polypyrrole Titanium with Long-Term Stability and Potential Osteoinductive Ability

被引：57

作者：

Chen, Lifeng ^{[1
]}

Mou, Shan ^{[1
]}

Li, Fangying ^{[3
]}

Zeng, Yuyang ^{[1
]}

Sun, Yang ^{[2
]}

Horch, Raymund E. ^{[4
,5
]}

Wei, Wei ^{[3
]}

Wang, Zhenxing ^{[1
]}

Sun, Jiaming ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Dept Plast Surg, Wuhan 430022, Hubei, Peoples R China

[2] Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Dept Med Records Management & Stat, Wuhan 430022, Hubei, Peoples R China

[3] Wuhan Text Univ, Hubei Int Sci & Technol Cooperat Base Intelligent, Hubei Key Lab Adv Text Mat & Applicat, Wuhan 430200, Hubei, Peoples R China

[4] Friedrich Alexander Univ Erlangen Nuenberg, Univ Hosp Erlangen, Dept Plast & Hand Surg, FAU, Schlosspl 4, D-91054 Erlangen, Bavaria, Germany

[5] Friedrich Alexander Univ Erlangen Nuenberg, Univ Hosp Erlangen, Lab Tissue Engn & Regenerat Med, FAU, Schlosspl 4, D-91054 Erlangen, Bavaria, Germany

来源：

ACS APPLIED MATERIALS & INTERFACES | 2019年 / 11卷 / 49期

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

polypyrrole; adipose stem cell-derived extracellular vesicles; biotin-streptavidin complex; surface modification; osteoinductivity; SURFACE FUNCTIONALIZATION; STRATEGY; DIFFERENTIATION; PROTEINS;

D O I：

10.1021/acsami.9b17015

中图分类号：

TB3 [工程材料学];

学科分类号：

082905 [生物质能源与材料];

摘要：

Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs), containing proteins or microRNAs (miRNAs), possessing various biological activity and low immunogenicity, are considered promising for surface modification of bone grafts. However, the modification efficiency is not satisfied yet, resulting in compromised therapy effects. Here, we report a novel immobilized method by self-assembling biotinylated MSCEVs onto the surface of biotin-doped polypyrrole titanium (Bio-Ppy-Ti) to improve its biofunctions in vitro and in vivo. Using this method, the amount of human adipose derived stem cell-EVs (hASC-EVs) anchored onto the Bio-Ppy-Ti surface was 185-fold higher than that of pure Ti after ultrasonic concussion for 30 s and it remained stable on the Bio-Ppy-Ti surface for 14 days at 4 degrees C. Compared to pristine Ti, EV-Bio-Ppy-Ti exhibited enhanced cell compatibility and osteoinductivity for osteoblasts in vitro and antiapoptosis ability in the ectopic bone formation mode. Gene chip analysis further demonstrated that several osteoinductive miRNAs were encapsulated in hASC-EVs, which may explain the high bone regeneration ability of EV-Bio-Ppy-Ti. Thus, this MSC-EV biotin-immobilized method appears to be highly efficient and long-term stable for demonstrating its potential for clinical metal implants.

引用

页码：46183 / 46196

页数：14

共 48 条

[11]

Classification of osseointegrated implant surfaces: materials, chemistry and topography [J].