Femtosecond transient absorption study of the dynamics of acrylodan in solution and attached to human serum albumin

被引：18

作者：

Buzády, A

Savolainen, J

Erostyák, J

Myllyperkiö, P

Somogyi, B

Korppi-Tommola, J

机构：

[1] Univ Pecs, Inst Phys, Dept Expt Phys, H-7624 Pecs, Hungary

[2] Univ Pecs, Dept Biophys, H-7625 Pecs, Hungary

[3] Univ Jyvaskyla, Dept Chem, FIN-40351 Jyvaskyla, Finland

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 2003年 / 107卷 / 05期

关键词：

D O I：

10.1021/jp027107o

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The excited-state relaxation dynamics of the protein-labeling dye acrylodan in solution and attached to human serum albumin has been studied by femtosecond transient absorption spectroscopy. Time-resolved spectra and kinetics of stimulated emission and excited-state absorption in the wavelength region from 400 to 800 nm were studied in ethanol and dimethylformamide. The excited-state solvation dynamics is characterized by multiexponential behavior in both solvents. In ethanol solution, the time dependence of the transient spectra is interpreted in terms of fast solvent relaxation followed by excited-state isomerization of the dye. Acrylodan attached to the protein shows a relaxation component of 3 ps that is attributed to the solvation dynamics of water in the neighborhood of the fluorescent label.

引用

页码：1208 / 1214

页数：7

共 34 条

[1]

Baker GA, 2001, BIOPOLYMERS, V59, P502, DOI 10.1002/1097-0282(200112)59:7<502::AID-BIP1055>3.0.CO

[2]

2-I

[3] Phase-fluorimetry study on dielectric relaxation of human serum albumin [J].

Buzády, A ;

Erostyák, J ;

Somogyi, B .

BIOPHYSICAL CHEMISTRY, 2000, 88 (1-3) :153-163

[4] Phase-fluorometry study on dielectric relaxation of acrylodan-labeled human serum albumin [J].

Buzády, A ;

Erostyák, J ;

Somogyi, B .

BIOPHYSICAL CHEMISTRY, 2001, 94 (1-2) :75-85

[5] SUBPICOSECOND RESOLUTION STUDIES OF SOLVATION DYNAMICS IN POLAR APROTIC AND ALCOHOL SOLVENTS [J].

CASTNER, EW ;

MARONCELLI, M ;

FLEMING, GR .

JOURNAL OF CHEMICAL PHYSICS, 1987, 86 (03) :1090-1097

[6] Ultrafast dielectric response of proteins from dynamics stokes shifting of coumarin in calmodulin [J].

Changenet-Barret, P ;

Choma, CT ;

Gooding, EF ;

DeGrado, WF ;

Hochstrasser, RM .

JOURNAL OF PHYSICAL CHEMISTRY B, 2000, 104 (39) :9322-9329

[7] Fatty acid binding to human serum albumin: new insights from crystallographic studies [J].

Curry, S ;

Brick, P ;

Franks, NP .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 1999, 1441 (2-3) :131-140

[8] Solvation dynamics of coumarin 480 in vesicles [J].

Datta, A ;

Pal, SK ;

Mandal, D ;

Bhattacharya, K .

JOURNAL OF PHYSICAL CHEMISTRY B, 1998, 102 (31) :6114-6117

[9] Chromophore-solvent dynamics [J].

Fleming, GR ;

Cho, MH .

ANNUAL REVIEW OF PHYSICAL CHEMISTRY, 1996, 47 :109-134

[10] Unfolding of acrylodan-labeled human serum albumin probed by steady-state and time-resolved fluorescence methods [J].

Flora, K ;

Brennan, JD ;

Baker, GA ;

Doody, MA ;

Bright, FV .

BIOPHYSICAL JOURNAL, 1998, 75 (02) :1084-1096

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