Soluble adhesion molecules (sICAM-1, sL-Selectin, sE-Selectin, sCD44) in healthy allogenic peripheral stem-cell donors primed with recombinant C-CSF

被引:7
作者
Arslan, Ö [1 ]
Akan, H [1 ]
Arat, M [1 ]
Dalva, K [1 ]
Özcan, M [1 ]
Gürman, G [1 ]
Ilhan, O [1 ]
Konuk, N [1 ]
Beksaç, M [1 ]
Uysal, A [1 ]
Koç, H [1 ]
机构
[1] Ankara Univ, Ibni Sina Hosp, Sch Med, Dept Hematol, TR-06100 Ankara, Turkey
关键词
soluble adhesion molecules; allogeneic stem cell transplantation; stem cell mobilization; normal donors; engraftment;
D O I
10.1080/146532400539198
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Background We analysed the effects of rhG-CSF (Amgen-Roche, USA) on serum changes of four soluble adhesion molecules (SAM) (sICAM-1, sL-Selectin, sE-Selectin and sCD44) in healthy peripheral allogeneic stem-cell transplantation donors and their correlation with acute GvHD and effect on engraftment kinetics. Methods Serum SAM of 15 consecutive healthy HLA identical-sibling donors (median age 30 years, male:female ratio 7:8) were monitored using a commercial ELISA Kit (Bender Med, Austria) prior to, on the day of first apheresis and 24 h after the cessation of rhG-CSF (10 mug/kg/day s.c. on 5 days) administration. Leukapheresis was started on the fifth day of rhG-CSF administration, using a continuous-flow blood separator (Cobe Spectra, COBE BCT, Inc, Lakewood CO). Apheresis cycles were continued daily until a target of 4.0 x 10(6) CD34(+) cells/kg was reached. Results The results indicate a steady rise of sL-Selectin, sE-Selectin, and sCD44, but not of sICAM-1. Median number of mononuclear cells (MNC) and CD34(+) cells transfused were 7.7 x 10(8)/kg and 6.0 x 10(6)/kg, respectively. There was a near-significant correlation between the sL-Selectin levels and CD34(+) cell yield (r = 0.49, 0.06). Median granulocyte and platelet engraftment days were 11 (10-18) and 12 (9-33), respectively. There was a significant inverse correlation between the CD34(+) cell dose and granulocyte levels (r = -0.68, p = 0.022), but not for platelet engraftment. The only correlation between SAM levels and engraftment war for sICAM-1 levels increasing sICAM-1 levels were a sign of prolonged neutropenia (r = 0.72,p = 0.011). No correlation between the apheresis day serum levels of adhesion molecules and acute GVHD was documented. Discussion Analysis of sICAM-1, sL-Selectin, sE-Selectin and sCD44 levels during allogeneic PBSC apheresis did not reveal any significant effect on engraftment and GvHD, except the correlation of sL-Selectin levels and collected CD34(+) cells. More research and data about the role of not only SAM levels, bat also antigenic expression of SAM are required to enlighten leukocyte-endothelial cell interactions and egress of stem cells during G-CSF administration.
引用
收藏
页码:259 / 265
页数:7
相关论文
共 18 条
  • [11] Anti-VLA4/VCAM-1-induced mobilization requires cooperative signaling through the kit/mkit ligand pathway
    Papayannopoulou, T
    Priestley, GV
    Nakamoto, B
    [J]. BLOOD, 1998, 91 (07) : 2231 - 2239
  • [12] PRZEPIORKA D, 1995, BONE MARROW TRANSPL, V15, P825
  • [13] SOLUBLE L-SELECTIN IS PRESENT IN HUMAN PLASMA AT HIGH-LEVELS AND RETAINS FUNCTIONAL-ACTIVITY
    SCHLEIFFENBAUM, B
    SPERTINI, O
    TEDDER, TF
    [J]. JOURNAL OF CELL BIOLOGY, 1992, 119 (01) : 229 - 238
  • [14] PRIMARY TRANSPLANTATION OF ALLOGENEIC PERIPHERAL-BLOOD PROGENITOR CELLS MOBILIZED BY FILGRASTIM (GRANULOCYTE-COLONY-STIMULATING FACTOR)
    SCHMITZ, N
    DREGER, P
    SUTTORP, M
    ROHWEDDER, EB
    HAFERLACH, T
    LOFFLER, H
    HUNTER, A
    RUSSELL, NH
    [J]. BLOOD, 1995, 85 (06) : 1666 - 1672
  • [15] SIMMONS PJ, 1992, BLOOD, V80, P388
  • [16] ELISA FOR QUANTITATION OF L-SELECTIN SHED FROM LEUKOCYTES INVIVO
    SPERTINI, O
    SCHLEIFFENBAUM, B
    WHITEOWEN, C
    RUIZ, P
    TEDDER, TF
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1992, 156 (01) : 115 - 123
  • [17] ROLE OF BETA-1-INTEGRINS AND BETA-2-INTEGRINS IN THE ADHESION OF HUMAN CD34HI STEM-CELLS TO BONE-MARROW STROMA
    TEIXIDO, J
    HEMLER, ME
    GREENBERGER, JS
    ANKLESARIA, P
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1992, 90 (02) : 358 - 367
  • [18] TO LB, 1994, BLOOD, V84, P2930