PGC-1 and PERC, coactivators of the estrogen receptor-related receptor γ

被引：62

作者：

Hentschke, M ^{[1
]}

Süsens, U ^{[1
]}

Borgmeyer, U ^{[1
]}

机构：

[1] Univ Hamburg, Inst Entwicklungsneurobiol, Zentrum Mol Neurobiol, D-20246 Hamburg, Germany

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 299卷 / 05期

关键词：

transcription factor; ERR3; NR3B3; nuclear localization; EGFP; transactivation; pull down;

D O I：

10.1016/S0006-291X(02)02753-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mouse nuclear receptor ERRgamma (estrogen receptor-related receptor 7) is highly expressed in heart, skeletal muscle, kidney, and brain, as well as in the developing nervous system. We found that the expression of the coactivators PGC-1 (PGC-1alpha) and PERC (PGC-1beta) in mammalian cells augmented potently the transcriptional activation by ERRgamma. The constitutive activation function 2 (AF-2) of the orphan receptor was important for the synergistic enhancement. Functional receptor truncation analysis revealed an additional amino-terminal activation function, specific for the ERRgamma2 isoform and PGC-1. In vitro experiments showed a direct interaction of ERRgamma with both coactivators. Our findings suggest distinct regulatory functions for PGG-1 and PERC as tissue-specific coactivators for ERRgamma. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：872 / 879

页数：8

共 42 条

[1] ROLE OF THE 2 ACTIVATING DOMAINS OF THE ESTROGEN-RECEPTOR IN THE CELL-TYPE AND PROMOTER-CONTEXT DEPENDENT AGONISTIC ACTIVITY OF THE ANTIESTROGEN 4-HYDROXYTAMOXIFEN [J].

BERRY, M ;

METZGER, D ;

CHAMBON, P .

EMBO JOURNAL, 1990, 9 (09) :2811-2818

[2]

Chambon Pierre, 1994, Seminars in Cell Biology, V5, P115, DOI 10.1006/scel.1994.1015

[3] Identification of two hERR2-related novel nuclear receptors utilizing bioinformatics and inverse PCR [J].

Chen, F ;

Zhang, Q ;

McDonald, T ;

Davidoff, MJ ;

Wendy, B ;

Bai, C ;

Liu, QY ;

Caskey, CT .

GENE, 1999, 228 (1-2) :101-109

[4] Isolation of a gene encoding a novel member of the nuclear receptor superfamily from the critical region of usher syndrome type IIa at 1q41 [J].

Eudy, JD ;

Yao, SF ;

Weston, MD ;

Ma-Edmonds, M ;

Talmadge, CB ;

Cheng, JJ ;

Kimberling, WJ ;

Sumegi, J .

GENOMICS, 1998, 50 (03) :382-384

[5] To ERR in the estrogen pathway [J].

Giguère, V .

TRENDS IN ENDOCRINOLOGY AND METABOLISM, 2002, 13 (05) :220-225

[6] IDENTIFICATION OF A NEW CLASS OF STEROID-HORMONE RECEPTORS [J].

GIGUERE, V ;

YANG, N ;

SEGUI, P ;

EVANS, RM .

NATURE, 1988, 331 (6151) :91-94

[7] NUCLEAR RECEPTORS ENHANCE OUR UNDERSTANDING OF TRANSCRIPTION REGULATION [J].

GREEN, S ;

CHAMBON, P .

TRENDS IN GENETICS, 1988, 4 (11) :309-314

[8] Structural and functional evidence for ligand-independent transcriptional activation by the estrogen-related receptor 3 [J].

Greschik, H ;

Wurtz, JM ;

Sanglier, S ;

Bourguet, W ;

van Dorsselaer, A ;

Moras, D ;

Renaud, JP .

MOLECULAR CELL, 2002, 9 (02) :303-313

[9] PPARδ is an APC-regulated target of nonsteroidal anti-inflammatory drugs [J].

He, TC ;

Chan, TA ;

Vogelstein, B ;

Kinzler, KW .

CELL, 1999, 99 (03) :335-345

[10] Human ERRγ, a third member of the estrogen receptor-related receptor (ERR) subfamily of orphan nuclear receptors: Tissue-specific isoforms are expressed during development and in the adult [J].

Heard, DJ ;

Norby, PL ;

Holloway, J ;

Vissing, H .

MOLECULAR ENDOCRINOLOGY, 2000, 14 (03) :382-392

← 1 2 3 4 5 →