RAD marker microarrays enable rapid mapping of zebrafish mutations

被引：44

作者：

Miller, Michael R.

Atwood, Tressa S.

Eames, B. Frank

Eberhart, Johann K.

Yan, Yi-Lin

Postlethwait, John H.

Johnson, Eric A.

机构：

[1] Univ Oregon, Inst Mol Biol, Eugene, OR 97403 USA

[2] Univ Oregon, Inst Neurosci, Eugene, OR 97403 USA

[3] FloraGenex Inc, Eugene, OR 97403 USA

来源：

GENOME BIOLOGY | 2007年 / 8卷 / 06期

关键词：

D O I：

10.1186/gb-2007-8-6-r105

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We constructed a restriction site associated DNA ( RAD) marker microarray to facilitate rapid genetic mapping of zebrafish mutations. Using these microarrays with a bulk segregant approach, we localized previously unmapped mutations to genomic regions just a few centiMorgans in length. Furthermore, we developed an approach to assay individual RAD markers in pooled populations and refined one region. The RAD approach is highly effective for genetic mapping in zebrafish and is an attractive option for mapping in other organisms.

引用

页数：10

共 25 条

[1] Zebrafish: A genetic model for vertebrate organogenesis and human disorders
Ackermann, GE
Paw, BH
[J]. FRONTIERS IN BIOSCIENCE-LANDMARK, 2003, 8 : D1227 - D1253
[2] Bahary N, 2004, METHOD CELL BIOL, V77, P305
[3] Making waves in cancer research: new models in the zebrafish
Berghmans, S
Jette, C
Langenau, D
Hsu, K
Stewart, R
Look, T
Kanki, JP
[J]. BIOTECHNIQUES, 2005, 39 (02) : 227 - 237
[4] Ensembl 2006
Birney, E.
Andrews, D.
Caccamo, M.
Chen, Y.
Clarke, L.
Coates, G.
Cox, T.
Cunningham, F.
Curwen, V.
Cutts, T.
Down, T.
Durbin, R.
Fernandez-Suarez, X. M.
Flicek, P.
Graf, S.
Hammond, M.
Herrero, J.
Howe, K.
Iyer, V.
Jekosch, K.
Kahari, A.
Kasprzyk, A.
Keefe, D.
Kokocinski, F.
Kulesha, E.
London, D.
Longden, I.
Melsopp, C.
Meidl, P.
Overduin, B.
Parker, A.
Proctor, G.
Prlic, A.
Rae, M.
Rios, D.
Redmond, S.
Schuster, M.
Sealy, I.
Searle, S.
Severin, J.
Slater, G.
Smedley, D.
Smith, J.
Stabenau, A.
Stalker, J.
Trevanion, S.
Ureta-Vidal, A.
Vogel, J.
White, S.
Woodwark, C.
[J]. NUCLEIC ACIDS RESEARCH, 2006, 34 : D556 - D561
[5] Zebrafish as a genomics research model
Chen, E
Ekker, SC
[J]. CURRENT PHARMACEUTICAL BIOTECHNOLOGY, 2004, 5 (05) : 409 - 413
[6] An integrin-dependent role of pouch endoderm in hyoid cartilage development
Crump, JG
Swartz, ME
Kimmel, CB
[J]. PLOS BIOLOGY, 2004, 2 (09): : 1432 - 1445
[7] TOUCHDOWN PCR TO CIRCUMVENT SPURIOUS PRIMING DURING GENE AMPLIFICATION
DON, RH
COX, PT
WAINWRIGHT, BJ
BAKER, K
MATTICK, JS
[J]. NUCLEIC ACIDS RESEARCH, 1991, 19 (14) : 4008 - 4008
[8] Driever W, 1996, DEVELOPMENT, V123, P37
[9] Zebrafish as a pharmacological tool: the how, why and when
Goldsmith, P
[J]. CURRENT OPINION IN PHARMACOLOGY, 2004, 4 (05) : 504 - 512
[10] Genetic variation in the zebrafish
Guryev, V
Koudijs, MJ
Berezikov, E
Johnson, SL
Plasterk, RHA
van Eeden, FJM
Cuppen, E
[J]. GENOME RESEARCH, 2006, 16 (04) : 491 - 497

← 1 2 3 →