RAD marker microarrays enable rapid mapping of zebrafish mutations

被引：44

作者：

Miller, Michael R.

Atwood, Tressa S.

Eames, B. Frank

Eberhart, Johann K.

Yan, Yi-Lin

Postlethwait, John H.

Johnson, Eric A.

机构：

[1] Univ Oregon, Inst Mol Biol, Eugene, OR 97403 USA

[2] Univ Oregon, Inst Neurosci, Eugene, OR 97403 USA

[3] FloraGenex Inc, Eugene, OR 97403 USA

来源：

GENOME BIOLOGY | 2007年 / 8卷 / 06期

关键词：

D O I：

10.1186/gb-2007-8-6-r105

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We constructed a restriction site associated DNA ( RAD) marker microarray to facilitate rapid genetic mapping of zebrafish mutations. Using these microarrays with a bulk segregant approach, we localized previously unmapped mutations to genomic regions just a few centiMorgans in length. Furthermore, we developed an approach to assay individual RAD markers in pooled populations and refined one region. The RAD approach is highly effective for genetic mapping in zebrafish and is an attractive option for mapping in other organisms.

引用

页数：10

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