Enhancement of Aggregation-Induced Emission in Dye-Encapsulating Polymeric Micelles for Bioimaging

被引:231
作者
Wu, Wen-Chung [1 ]
Chen, Ching-Yi [1 ]
Tian, Yanqing [1 ]
Jang, Sei-Hum [1 ]
Hong, Yuning [2 ]
Liu, Yang [2 ]
Hu, Rongrong [2 ]
Tang, Ben Zhong [2 ]
Lee, Yi-Ting [4 ]
Chen, Chin-Ti [4 ]
Chen, Wen-Chang [3 ]
Jen, Alex K. -Y. [1 ]
机构
[1] Univ Washington, Dept Mat Sci & Engn, Seattle, WA 98195 USA
[2] Hong Kong Univ Sci & Technol, Dept Chem, Kowloon, Hong Kong, Peoples R China
[3] Natl Taiwan Univ, Dept Chem Engn, Taipei 106, Taiwan
[4] Acad Sinica, Inst Chem, Taipei 115, Taiwan
基金
新加坡国家研究基金会; 美国国家科学基金会;
关键词
MODIFIED SILICA NANOPARTICLES; BLOCK-COPOLYMER; ENERGY-TRANSFER; DRUG-DELIVERY; IN-VIVO; FLUORESCENCE; MOLECULES; CELL; PHOTOLUMINESCENCE; AUTOFLUORESCENCE;
D O I
10.1002/adfm.200902043
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Three amphiphilic block copolymers are employed to form polymeric micelles and function as nanocarriers to disperse hydrophobic aggregation-induced emission (AIE) dyes, 1,1,2,3,4,5-hexaphenylsilole (HPS) and/or bis(4-(N-(1-naphthyl) phenylamino)-phenyl)fumaronitrile (NPAFN), into aqueous solution for biological studies. Compared to their virtually non-emissive properties in organic solutions, the fluorescence intensity of these AIE dyes has increased significantly due to the spatial confinement that restricts intramolecular rotation of these dyes and their better compatibility in the hydrophobic core of polymeric micelles. The effect of the chemical structure of micelle cores on the photophysical properties of AIE dyes are investigated, and the fluorescence resonance energy transfer (FRET) from the green-emitting donor (HPS) to the red-emitting acceptor (NPAFN) is explored by co-encapsulating this FRET pair in the same micelle core. The highest fluorescence quantum yield (similar to 62%) could be achieved by encapsulating H PS aggregates in the micelles. Efficient energy transfer (>99%) and high amplification of emission (as high as 8 times) from the NPAFN acceptor could also be achieved by spatially confining the HPS/NPAFN FRET pair in the hydrophobic core of polymeric micelles. These micelles could be successfully internalized into the RAW 264.7 cells to demonstrate high-quality fluorescent images and cell viability due to improved quantum yield and reduced cytotoxicity.
引用
收藏
页码:1413 / 1423
页数:11
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