Secretion of the sweet-tasting protein thaumatin by recombinant strains of Aspergillus niger var. awamori

被引:25
作者
Faus, I
del Moral, C
Adroer, N
del Río, JL
Patiño, C
Sisniega, H
Casas, C
Bladé, J
Rubio, V
机构
[1] URQUIMA SA, URIACH Grp, E-08015 Barcelona, Spain
[2] Univ Autonoma Madrid, CSIC, Ctr Nacl Biotecnol, E-28049 Madrid, Spain
[3] Univ Autonoma Barcelona, Fac Ciencies, Unitat Engn Quim, Bellaterra 08193, Barcelona, Spain
关键词
D O I
10.1007/s002530051188
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A recombinant form of the sweet-tasting protein thaumatin has been produced in the filamentous fungus Aspergillus niger var. awamori. Expression cassettes containing a synthetic gene encoding thaumatin II were prepared and used to transform Aspergillus niger var, awamori strain NRRL312. Several fungal strains capable of synthesizing and secreting thaumatin into the culture medium were generated, and their production capabilities were determined, first in shake flasks and later in a laboratory fermenter. We report the expression and secretion of thaumatin in concentrations of 5-7 mg/l. This recombinant thaumatin is sweet.
引用
收藏
页码:393 / 398
页数:6
相关论文
共 18 条
  • [1] ALLEN HJ, 1992, GLYCONJUGATES COMPOS, P167
  • [2] THE DEVELOPMENT OF ASPERGILLUS-NIGER VAR AWAMORI AS A HOST FOR THE EXPRESSION AND SECRETION OF HETEROLOGOUS GENE-PRODUCTS
    BERKA, RM
    KODAMA, KH
    REY, MW
    WILSON, LJ
    WARD, M
    [J]. BIOCHEMICAL SOCIETY TRANSACTIONS, 1991, 19 (03) : 681 - 685
  • [3] SECRETION OF HETEROLOGOUS PROTEINS BY ASPERGILLUS-NIGER - PRODUCTION OF ACTIVE HUMAN INTERLEUKIN-6 IN A PROTEASE-DEFICIENT MUTANT BY KEX2-LIKE PROCESSING OF A GLUCOAMYLASE-HIL6 FUSION PROTEIN
    BROEKHUIJSEN, MP
    MATTERN, IE
    CONTRERAS, R
    KINGHORN, JR
    VANDENHONDEL, CAMJJ
    [J]. JOURNAL OF BIOTECHNOLOGY, 1993, 31 (02) : 135 - 145
  • [4] EFFICIENT KEX2-LIKE PROCESSING OF A GLUCOAMYLASE-INTERLEUKIN-6 FUSION PROTEIN BY ASPERGILLUS-NIDULANS AND SECRETION OF MATURE INTERLEUKIN-6
    CONTRERAS, R
    CARREZ, D
    KINGHORN, JR
    VANDENHONDEL, CAMJJ
    FIERS, W
    [J]. BIO-TECHNOLOGY, 1991, 9 (04): : 378 - 381
  • [5] COMMERCIAL LEVELS OF CHYMOSIN PRODUCTION BY ASPERGILLUS
    DUNNCOLEMAN, NS
    BLOEBAUM, P
    BERKA, RM
    BODIE, E
    ROBINSON, N
    ARMSTRONG, G
    WARD, M
    PRZETAK, M
    CARTER, GL
    LACOST, R
    WILSON, LJ
    KODAMA, KH
    BALIU, EF
    BOWER, B
    LAMSA, M
    HEINSOHN, H
    [J]. BIO-TECHNOLOGY, 1991, 9 (10): : 976 - 981
  • [6] Expression of a synthetic gene encoding the sweet-tasting protein thaumatin in Escherichia coli
    Faus, I
    Patino, C
    delRio, JL
    delMoral, C
    Barroso, HS
    Rubio, V
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 229 (01) : 121 - 127
  • [7] Expression of a synthetic gene encoding the sweet-tasting protein thaumatin in the filamentous fungus Penicillium roquefortii
    Faus, I
    Patiño, C
    del Río, JL
    del Moral, C
    Barroso, HS
    Bladé, J
    Rubio, V
    [J]. BIOTECHNOLOGY LETTERS, 1997, 19 (12) : 1185 - 1191
  • [8] REGULATION OF THE GLAA GENE OF ASPERGILLUS-NIGER
    FOWLER, T
    BERKA, RM
    WARD, M
    [J]. CURRENT GENETICS, 1990, 18 (06) : 537 - 545
  • [9] Jeenes D J, 1991, Biotechnol Genet Eng Rev, V9, P327
  • [10] JEENES DJ, 1993, FEMS MICROBIOL LETT, V107, P267