Voltage-gated mobility of the Ca2+ channel cytoplasmic tails and its regulatory role

被引：46

作者：

Kobrinsky, E ^{[1
]}

Schwartz, E ^{[1
]}

Abernethy, DR ^{[1
]}

Soldatov, NM ^{[1
]}

机构：

[1] NIA, NIH, Baltimore, MD 21224 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2003年 / 278卷 / 07期

关键词：

D O I：

10.1074/jbc.M211254200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transient increase in intracellular free Ca2+ concentration generated by the voltage-gated Ca(v)1.2 channels acts as an important intracellular signal. By using fluorescence resonance energy transfer combined with patch clamp in living cells, we present evidence for voltage-gated mobility of the cytoplasmic tails of the Ca(v)1.2 channel and for its regulatory role in intracellular signaling. Anchoring of the C-terminal tail to the plasma membrane caused an inhibition of its state-dependent mobility, channel inactivation, and CREB-dependent transcription. Release of the tail restored these functions suggesting a direct role for voltage-gated mobility of the C-terminal tail in Ca2+ signaling.

引用

页码：5021 / 5028

页数：8

共 43 条

[41] Inhibition of T cell receptor-coreceptor interactions by antagonist ligands visualized by live FRET imaging of the T-hybridoma immunological synapse [J].