Direct and in vitro observation of growth hormone receptor molecules in A549 human lung epithelial cells by nanodiamond labeling

被引：97

作者：

Cheng, C.-Y. ^{[1
]}

Perevedentseva, E.

Tu, J.-S.

Chung, P.-H.

Cheng, C.-L.

Liu, K.-K.

Chao, J.-I.

Chen, P.-H.

Chang, C.-C.

机构：

[1] Natl Dong Hwa Univ, Dept Phys, Hualien 97401, Taiwan

[2] Tzu Chi Univ, Inst Pharmacol & Toxicol, Hualien 970, Taiwan

[3] Natl Chiao Tung Univ, Dept Biol Sci & Technol, Hsinchu 30050, Taiwan

[4] Acad Sinica, Inst Phys, Taipei 11529, Taiwan

[5] Natl Nano Device Lab, Hsinchu 30078, Taiwan

来源：

APPLIED PHYSICS LETTERS | 2007年 / 90卷 / 16期

关键词：

D O I：

10.1063/1.2727557

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

This letter presents direct observation of growth hormone receptor in one single cancer cell using nanodiamond-growth hormone complex as a specific probe. The interaction of surface growth hormone receptor of A549 human lung epithelial cells with growth hormone was observed using nanodiamond's unique spectroscopic signal via confocal Raman mapping. The growth hormone molecules were covalent conjugated to 100 nm diameter carboxylated nanodiamonds, which can be recognized specifically by the growth hormone receptors of A549 cell. The Raman spectroscopic signal of diamond provides direct and in vitro observation of growth hormone receptors in physiology condition in a single cell level. (C) 2007 American Institute of Physics.

引用

页数：3