Schizosaccharomyces pombe apn1 encodes a homologue of the Escherichia coli endonuclease IV family of DNA repair proteins

被引：25

作者：

Ramotar, D ^{[1
]}

Vadnais, J ^{[1
]}

Masson, JY ^{[1
]}

Tremblay, S ^{[1
]}

机构：

[1] Maisonneuve Rosemont Hosp, Res Ctr, Quebec City, PQ H1T 2M4, Canada

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1998年 / 1396卷 / 01期

关键词：

AP endonuclease; DNA repair; yeast;

D O I：

10.1016/S0167-4781(97)00160-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Apn1 protein of the budding yeast Saccharomyces cerevisiae is a DNA repair enzyme that hydrolyzes apurinic/apyrimidinic (AP) sites and removes 3'-blocking groups present at single strand breaks of damaged DNA. Yeast cells lacking Apn1 are hypersensitive to DNA damaging agents that produce AP sites and DNA strand breaks with blocked 3'-termini. In this study, we showed that the fission yeast Schizosaccharomyces pombe bears a homologue, Spapn1, that is 45% identical to S. cerevisiae Apn1. However, the Spapn1 gene is apparently not expressed. Active expression of S. cerevisiae Apn1 in S. pombe conferred no additional resistance to DNA damaging agents. These data suggest that the pathway by which S. pombe repairs AP sites is independent of a functional Apn1-like AP endonuclease. (C) 1998 Elsevier Science B.V.

引用

页码：15 / 20

页数：6

共 33 条

[11] DNA DAMAGE AND OXYGEN RADICAL TOXICITY [J].

IMLAY, JA ;

LINN, S .

SCIENCE, 1988, 240 (4857) :1302-1309

[12] SPECIFICITY OF THE MUTATOR CAUSED BY DELETION OF THE YEAST STRUCTURAL GENE (APN1) FOR THE MAJOR APURINIC ENDONUCLEASE [J].

KUNZ, BA ;

HENSON, ES ;

ROCHE, H ;

RAMOTAR, D ;

NUNOSHIBA, T ;

DEMPLE, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (17) :8165-8169

[13] ANALYSIS OF CLASS-II (HYDROLYTIC) AND CLASS-I (BETA-LYASE) APURINIC APYRIMIDINIC ENDONUCLEASES WITH A SYNTHETIC DNA SUBSTRATE [J].

LEVIN, JD ;

DEMPLE, B .

NUCLEIC ACIDS RESEARCH, 1990, 18 (17) :5069-5075

[14] INSTABILITY AND DECAY OF THE PRIMARY STRUCTURE OF DNA [J].

LINDAHL, T .

NATURE, 1993, 362 (6422) :709-715

[15] MUTAGENESIS BY APURINIC APYRIMIDINIC SITES [J].

LOEB, LA ;

PRESTON, BD .

ANNUAL REVIEW OF GENETICS, 1986, 20 :201-230

[16] The Schizosaccharomyces pombe spqM gene is a new member of the Qm transcription factor family [J].

Masson, JY ;

Vadnais, J ;

Ramotar, D .

GENE, 1996, 170 (01) :153-154

[17] Normal processing of AP sites in Apn1-deficient Saccharomyces cerevisiae is restored by Escherichia coli genes expressing either exonuclease III or endonuclease III [J].

Masson, JY ;

Ramotar, D .

MOLECULAR MICROBIOLOGY, 1997, 24 (04) :711-721

[18]

MASSON JY, 1996, IN PRESS GENE

[19] YEAST STRUCTURAL GENE (APN1) FOR THE MAJOR APURINIC ENDONUCLEASE - HOMOLOGY TO ESCHERICHIA-COLI ENDONUCLEASE-IV [J].

POPOFF, SC ;

SPIRA, AI ;

JOHNSON, AW ;

DEMPLE, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (11) :4193-4197

[20] ARCHITECTURAL FEATURES OF PRE-MESSENGER-RNA INTRONS IN THE FISSION YEAST SCHIZOSACCHAROMYCES-POMBE [J].

PRABHALA, G ;

ROSENBERG, GH ;

KAUFER, NF .

YEAST, 1992, 8 (03) :171-182

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