Immunity and other defenses in pea aphids, Acyrthosiphon pisum

被引:365
作者
Gerardo, Nicole M. [1 ]
Altincicek, Boran [2 ]
Anselme, Caroline [3 ,4 ]
Atamian, Hagop [5 ]
Barribeau, Seth M. [1 ]
De Vos, Martin [6 ]
Duncan, Elizabeth J. [7 ]
Evans, Jay D. [8 ]
Gabaldon, Toni [9 ]
Ghanim, Murad [10 ]
Heddi, Adelaziz [3 ]
Kaloshian, Isgouhi [5 ]
Latorre, Amparo [11 ,12 ,13 ]
Moya, Andres [11 ,12 ,13 ]
Nakabachi, Atsushi [14 ]
Parker, Benjamin J. [1 ]
Perez-Brocal, Vincente [3 ,11 ,12 ,13 ]
Pignatelli, Miguel [11 ,12 ,13 ]
Rahbe, Yvan [3 ]
Ramsey, John S. [6 ]
Spragg, Chelsea J. [1 ]
Tamames, Javier [11 ,12 ,13 ]
Tamarit, Daniel [11 ,12 ,13 ]
Tamborindeguy, Cecilia [15 ,16 ]
Vincent-Monegat, Caroline [3 ]
Vilcinskas, Andreas [2 ]
机构
[1] Emory Univ, Dept Biol, O Wayne Rollins Res Ctr, Atlanta, GA 30332 USA
[2] Univ Giessen, Inst Phytopathol & Appl Zool, Interdisciplinary Res Ctr, D-35392 Giessen, Germany
[3] Univ Lyon, INRA, BioEnvironm & Sante IFR41, INSA Lyon,BF2I UMR203, F-69621 Villeurbanne, France
[4] Univ Nice Sophia Antipolis, UMR Interact Biot & Sante Vegetale, INRA 1301, CNRS 6243, F-06903 Sophia Antipolis, France
[5] Univ Calif Riverside, Dept Nematol, Grad Program Genet Genom & Bioinformat, Riverside, CA 92521 USA
[6] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA
[7] Univ Otago, Dept Biochem, Lab Evolut & Dev, Dunedin 9054, New Zealand
[8] USDA ARS, Bee Res Lab, Beltsville, MD 20705 USA
[9] CRG, Bioinformat & Genom Programme, Barcelona 08003, Spain
[10] Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, Israel
[11] Univ Valencia, Inst Cavanilles Biodiversidad & Biol Evolut, Valencia 46071, Spain
[12] Conselleria Sanidad Gen Valenciana, CIBEResp, Valencia 46020, Spain
[13] Conselleria Sanidad Gen Valenciana, CSISP, Valencia 46020, Spain
[14] RIKEN, Adv Sci Inst, Wako, Saitama 3510198, Japan
[15] Cornell Univ, Plant Pathol & Plant Microbe Biol Dept, Ithaca, NY 14853 USA
[16] Texas A&M, Dept Entomol, College Stn, TX 77843 USA
来源
GENOME BIOLOGY | 2010年 / 11卷 / 02期
关键词
PEPTIDOGLYCAN RECOGNITION PROTEINS; MULTIPLE SEQUENCE ALIGNMENT; HUMORAL STRESS-RESPONSE; DROSOPHILA-MELANOGASTER; ANOPHELES-GAMBIAE; MOLECULAR CHARACTERIZATION; ANTIMICROBIAL PEPTIDES; BACTERIAL SYMBIONTS; REPRODUCTIVE EFFORT; EXPRESSION ANALYSIS;
D O I
10.1186/gb-2010-11-2-r21
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background: Recent genomic analyses of arthropod defense mechanisms suggest conservation of key elements underlying responses to pathogens, parasites and stresses. At the center of pathogen-induced immune responses are signaling pathways triggered by the recognition of fungal, bacterial and viral signatures. These pathways result in the production of response molecules, such as antimicrobial peptides and lysozymes, which degrade or destroy invaders. Using the recently sequenced genome of the pea aphid (Acyrthosiphon pisum), we conducted the first extensive annotation of the immune and stress gene repertoire of a hemipterous insect, which is phylogenetically distantly related to previously characterized insects models. Results: Strikingly, pea aphids appear to be missing genes present in insect genomes characterized to date and thought critical for recognition, signaling and killing of microbes. In line with results of gene annotation, experimental analyses designed to characterize immune response through the isolation of RNA transcripts and proteins from immune-challenged pea aphids uncovered few immune-related products. Gene expression studies, however, indicated some expression of immune and stress-related genes. Conclusions: The absence of genes suspected to be essential for the insect immune response suggests that the traditional view of insect immunity may not be as broadly applicable as once thought. The limitations of the aphid immune system may be representative of a broad range of insects, or may be aphid specific. We suggest that several aspects of the aphid life style, such as their association with microbial symbionts, could facilitate survival without strong immune protection.
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页数:16
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