The biosynthesis of indolocarbazoles in a heterologous E-coli host

被引：49

作者：

Hyun, CG

Bililign, T

Liao, JC

Thorson, JS

机构：

[1] Univ Wisconsin, Sch Pharm, Pharmceut Sci Div, Lab Biosynthet Chem, Madison, WI 53705 USA

[2] Cornell Univ, Joan & Sanford I Weill Grad Sch Med Sci, New York, NY 10021 USA

来源：

CHEMBIOCHEM | 2003年 / 4卷 / 01期

关键词：

biosynthesis; gene locus; heterologous expression; natural products; rebeccamycin;

D O I：

10.1002/cbic.200390004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The antitumor antibiotic rebeccamycin (1), produced by the bacterium Saccharothrix aerocolonigenes, is a prototype of a class of complex natural products called indolocarbazoles. The cloning and characterization of the rebeccamcyin (reb) gene locus from S. aerocolonigenes is reported. The heterologous expression of this intact gene cluster in Escherichia coli led to the production of 1 and two putative biosynthetic intermediates. This work represents the first production of indolocarbazole analogues in the heterologous host E. coli.

引用

页码：114 / 117

页数：4

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