Photocross-linking of nucleic acids to associated proteins

被引：216

作者：

Meisenheimer, KM

Koch, TH

机构：

[1] Dept. of Chemistry and Biochemistry, University of Colorado, Boulder

来源：

CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1997年 / 32卷 / 02期

关键词：

azide; aryl azide; 8-azidoadenosine; 8-azidoguanosine; 5-azidouridine; 5-bromo-2'-deoxyuridine; 5-bromouracil; 5-bromouridine; 5-iodocytidine; 5-iodo-2'-deoxyuridine; 5-iodouracil; 5-iodouridine; nucleoprotein; photocrosslinking; 4-thiouracil; 4-thiouridine; 4-thiothymidine; two-photon excitation;

D O I：

10.3109/10409239709108550

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Photocross-linking is a useful technique for the partial definition of the nucleic acid-protein interface of nucleoprotein complexes. It can be accomplished by one or two photon excitations of wild-type nucleoprotein complexes or by one photon excitation of nucleoprotein complexes bearing one or more substitutions with photoreactive chromophores. Chromophores that have been incorporated into nucleic acids for this purpose include aryl azides, 5-azidouracil, 8-azidoadenine, 8-azidoguanine, 4-thiouracil, 5-bromouracil, 5-iodouracil, and 5-iodocytosine. The various techniques and chromophores are described and compared, with attention to the photochemical mechanism.

引用

页码：101 / 140

页数：40

共 119 条

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