Structure and function of Cdc6/Cdc18: Implications for origin recognition and checkpoint control

被引：183

作者：

Liu, JY ^{[1
]}

Smith, CL ^{[1
]}

DeRyckere, D ^{[1
]}

DeAngelis, K ^{[1
]}

Martin, GS ^{[1
]}

Berger, JM ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Mol & Cell Biol, 229 Stanley Hall, Berkeley, CA 94720 USA

来源：

MOLECULAR CELL | 2000年 / 6卷 / 03期

关键词：

D O I：

10.1016/S1097-2765(00)00062-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cdc6/Cdc18 is a conserved and essential component of prereplication complexes. The 2.0 Angstrom crystal structure of an archaeal Cdc6 ortholog, in conjunction with a mutational analysis of the homologous Cdc18 protein from Schizosaccharomyces pombe, reveals novel aspects of Cdc6/Cdc18 function. Two domains of Cdc6 form an AAA(+)-type nucleotide binding fold that is observed bound to Mg ADP. A third domain adopts a winged-helix fold similar to known DNA binding modules. Sequence comparisons show that the winged-helix domain is conserved in Orc1, and mutagenesis data demonstrate that this region of Cdc6/Cdc18 is required for function in vivo. Additional mutational analyses suggest that nucleotide binding and/or hydrolysis by Cdc6/Cdc18 is required not only for progression through S phase, but also for maintenance of checkpoint control during S phase.

引用

页码：637 / 648

页数：12

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