Human Bocavirus Capsid Structure: Insights into the Structural Repertoire of the Parvoviridae
被引:70
作者:
Gurda, Brittney L.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Florida, Dept Biochem & Mol Biol, Gainesville, FL 32610 USAUniv Calif San Diego, Dept Chem & Biochem, San Diego, CA 92093 USA
Gurda, Brittney L.
[3
]
Parent, Kristin N.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Diego, Dept Chem & Biochem, San Diego, CA 92093 USAUniv Calif San Diego, Dept Chem & Biochem, San Diego, CA 92093 USA
Parent, Kristin N.
[1
]
Bladek, Heather
论文数: 0引用数: 0
h-index: 0
机构:
Univ Florida, Dept Biochem & Mol Biol, Gainesville, FL 32610 USAUniv Calif San Diego, Dept Chem & Biochem, San Diego, CA 92093 USA
Bladek, Heather
[3
]
Sinkovits, Robert S.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Diego, Dept Chem & Biochem, San Diego, CA 92093 USAUniv Calif San Diego, Dept Chem & Biochem, San Diego, CA 92093 USA
Sinkovits, Robert S.
[1
]
DiMattia, Michael A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Florida, Dept Biochem & Mol Biol, Gainesville, FL 32610 USAUniv Calif San Diego, Dept Chem & Biochem, San Diego, CA 92093 USA
DiMattia, Michael A.
[3
]
Rence, Chelsea
论文数: 0引用数: 0
h-index: 0
机构:
Univ Florida, Dept Biochem & Mol Biol, Gainesville, FL 32610 USAUniv Calif San Diego, Dept Chem & Biochem, San Diego, CA 92093 USA
Rence, Chelsea
[3
]
Castro, Alejandro
论文数: 0引用数: 0
h-index: 0
机构:
Univ Florida, Dept Biochem & Mol Biol, Gainesville, FL 32610 USAUniv Calif San Diego, Dept Chem & Biochem, San Diego, CA 92093 USA
Castro, Alejandro
[3
]
McKenna, Robert
论文数: 0引用数: 0
h-index: 0
机构:
Univ Florida, Dept Biochem & Mol Biol, Gainesville, FL 32610 USAUniv Calif San Diego, Dept Chem & Biochem, San Diego, CA 92093 USA
McKenna, Robert
[3
]
Olson, Norm
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Diego, Dept Chem & Biochem, San Diego, CA 92093 USAUniv Calif San Diego, Dept Chem & Biochem, San Diego, CA 92093 USA
Olson, Norm
[1
]
Brown, Kevin
论文数: 0引用数: 0
h-index: 0
机构:
Hlth Protect Agcy, Ctr Infect, Virus Reference Dept, London NW9 5HT, EnglandUniv Calif San Diego, Dept Chem & Biochem, San Diego, CA 92093 USA
Brown, Kevin
[4
]
Baker, Timothy S.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Diego, Dept Chem & Biochem, San Diego, CA 92093 USA
Univ Calif San Diego, Div Biol Sci, San Diego, CA 92093 USAUniv Calif San Diego, Dept Chem & Biochem, San Diego, CA 92093 USA
Baker, Timothy S.
[1
,2
]
Agbandje-McKenna, Mavis
论文数: 0引用数: 0
h-index: 0
机构:
Univ Florida, Dept Biochem & Mol Biol, Gainesville, FL 32610 USAUniv Calif San Diego, Dept Chem & Biochem, San Diego, CA 92093 USA
Agbandje-McKenna, Mavis
[3
]
机构:
[1] Univ Calif San Diego, Dept Chem & Biochem, San Diego, CA 92093 USA
[2] Univ Calif San Diego, Div Biol Sci, San Diego, CA 92093 USA
Human bocavirus (HBoV) was recently discovered and classified in the Bocavirus genus (family Parvoviridae, subfamily Parvovirinae) on the basis of genomic similarity to bovine parvovirus and canine minute virus. HBoV has been implicated in respiratory tract infections and gastroenteric disease in children worldwide, yet despite numerous epidemiological reports, there has been limited biochemical and molecular characterization of the virus. Reported here is the three-dimensional structure of recombinant HBoV capsids, assembled from viral protein 2 (VP2), at 7.9-angstrom resolution as determined by cryo-electron microscopy and image reconstruction. A pseudo-atomic model of HBoV VP2 was derived from sequence alignment analysis and knowledge of the crystal structure of human parvovirus B19 (genus Erythrovirus). Comparison of the HBoV capsid structure to that of parvoviruses from five separate genera demonstrates strong conservation of a beta-barrel core domain and an alpha-helix, from which emanate several loops of various lengths and conformations, yielding a unique surface topology that differs from the three already described for this family. The highly conserved core is consistent with observations for other single-stranded DNA viruses, and variable surface loops have been shown to confer the host-specific tropism and the diverse antigenic properties of this family.