Determination of paracetamol (acetaminophen) in different body fluids and organ samples after solid-phase extraction using HPLC and an immunological method

被引:26
作者
Pufal, E
Sykutera, M
Rochholz, G
Schütz, HW
Sliwka, K
Kaatsch, HJ
机构
[1] Univ Kiel, Inst Rechtsmed, D-24105 Kiel, Germany
[2] Akad Med Ludwika Rydygiera, Katedra & Zaklad Med Sadowej, PL-85094 Bydgoszcz, Poland
来源
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 2000年 / 367卷 / 06期
关键词
D O I
10.1007/s002160000420
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A solid-phase extraction method routinely used for serum samples was improved and applied to the qualitative and quantitative determination of paracetamol in different body fluids, e.g. blood, urine, cerebrospinal fluid, synovial fluid, vitreous humor, and in tissue samples. A very simple method showed best results: Body fluids were mixed with phenacetine as internal standard and phosphate buffer (pH 6.8). Then protein was precipitated using acetonitrile. After strong centrifugation the supernatant was transferred to a preconditioned Bakerbond C18-SPE-column. Elution with methanol without a prior washing step showed best recovery rates. The extracts were investigated using high-performance liquid chromatography with ultraviolet detection, a photometrical and an immunochemical method.
引用
收藏
页码:596 / 599
页数:4
相关论文
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