Genomic diversity among Streptococcus agalactiae isolates detected by a degenerate oligonucleotide-primed amplification assay

被引：21

作者：

Limansky, AS ^{[1
]}

Sutich, EG ^{[1
]}

Guardati, MC ^{[1
]}

Toresani, IE ^{[1
]}

Viale, AM ^{[1
]}

机构：

[1] Univ Nacl Rosario, Fac Ciencias Bioquim & Farmaceut, Dept Microbiol, Programa Multidiscipl Biol Expt,CONICET, RA-2000 Rosario, Santa Fe, Argentina

来源：

JOURNAL OF INFECTIOUS DISEASES | 1998年 / 177卷 / 05期

关键词：

D O I：

10.1086/515275

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A random-amplified polymorphic DNA assay using partially degenerate oligonucleotides as primers was used for the characterization of 78 epidemiologically related and unrelated clinical isolates of Streptococcus agalactiae belonging to different serotypes. Thirty distinct amplification profiles were obtained among 52 unrelated S. agalactiae isolates assigned to nine groups by serotyping (including 3 nontypeable strains), uncovering the extent of genomic heterogeneity existent within serotypes. This method was particularly useful in providing evidence for or against vertical transmission of a given clone of this microorganism, as well as for relapsing or reinfection in related cases, and suggested clonal relatedness between unrelated S. agalactiae isolates associated with some invasive infections. Thus, this simple methodology represents a suitable tool for the epidemiologic study of S. agalactiae infections.

引用

页码：1308 / 1313

页数：6

共 29 条

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