Current and prospective applications of metal ion-protein binding

被引：401

作者：

Ueda, EKM

Gout, PW

Morganti, L

机构：

[1] Inst Nucl & Energy Res, CNEN, IPEN, Dept Biotechnol, BR-05508900 Sao Paulo, Brazil

[2] British Columbia Canc Agcy, Dept Canc Endocrinol, Vancouver, BC V5Z 4E6, Canada

来源：

JOURNAL OF CHROMATOGRAPHY A | 2003年 / 988卷 / 01期

关键词：

immobilized metal affinity chromatography; reviews; affinity chromatography; proteins;

D O I：

10.1016/S0021-9673(02)02057-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Since immobilized metal ion affinity chromatography (IMAC) was first introduced, several variants of this method and many other metal affinity-based techniques have been devised. IMAC quickly established itself as a highly reliable purification procedure, showing rapid expansion in the number of preparative and analytical applications while not remaining confined to protein separation. It was soon applied to protein refolding (matrix-assisted refolding), evaluation of protein folding status, protein surface topography studies and biosensor development. In this review, applications in protein processing are described of IMAC as well as other metal affinity-based technologies. (C) 2002 Elsevier Science B.V. All rights reserved.

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页码：1 / 23

页数：23

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