Determination of vitamin K homologues by high-performance liquid chromatography with on-line photoreactor and peroxyoxalate chemiluminescence detection

被引：49

作者：

Ahmed, Sameh ^{[1
]}

Kishikawa, Naoya ^{[1
]}

Nakashima, Kenichiro ^{[1
]}

Kuroda, Naotaka ^{[1
]}

机构：

[1] Nagasaki Univ, Course Pharmaceut Sci, Grad Sch Biomed Sci, Nagasaki 8528521, Japan

来源：

ANALYTICA CHIMICA ACTA | 2007年 / 591卷 / 02期

关键词：

vitamin k; peroxyoxalate chemiluminescence detection; ultraviolet irradiation; high-perforinance liquid chromatography; hydrogen peroxide;

D O I：

10.1016/j.aca.2007.03.061

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive and highly selective high-performance liquid chromatography (HPLC) method was developed for the determination of vitamin K homologues including phylloquinone (PK), menaquinone-4 (MK-4) and menaquinone-7 (MK-7) in human plasma using post-column peroxyoxalate chemiluminescence (PO-CL) detection following on-line ultraviolet (UV) irradiation. The method was based on ultraviolet irradiation (254 nm, 15 W) of vitamin K to produce hydrogen peroxide and a fluorescent product at the same time, which can be determined with PO-CL detection. The separation of vitamin K by HPLC was accomplished isocratically on an ODS column within 35 min. The method involves the use of 2-methyl-3pentadecyl-1,4-naphthoquinone as an internal standard. The detection limits (signal-to-noise ratio = 3) were 32, 38 and 85 fmol for PK, MK-4 and MK-7, respectively. The recoveries of PK, MK-4 and MK-7 were greater than 82% and the inter- and intra-assay R.S.D. values were 1.9-5.4%. The sensitivity and selectivity of this method were sufficient for clinical and nutritional applications. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：148 / 154

页数：7

共 31 条

[21] SYNTHESIS AND EVALUATION OF ARYL OXALATES AS PEROXYOXALATE CHEMI-LUMINESCENCE REAGENTS [J].