Optical measurement of the viscoelastic and biochemical responses of living cells to mechanical perturbation

被引:31
作者
Lee, CH
Guo, CL
Wang, J [1 ]
机构
[1] Acad Sinica, Inst Atom & Mol Sci, Taipei 106, Taiwan
[2] Natl Taiwan Univ, Dept Elect Engn, Taipei 106, Taiwan
关键词
D O I
10.1364/OL.23.000307
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We have developed an optical method for real-time monitoring of cellular motion in a natural environment with nanometer resolution. From the motion driven by small optical forces, we measured dynamic viscoelastic responses of living cells in the linear reversible region. Cytoplasmic gel-to-sol transition that was due to the disruption of the actin-filament framework was detected, and a linear release of Ca2+ from intracellular storage that was related to submicrometer cell deformation was observed. The method was shown to be a powerful tool for studying the natural response of cells to mechanical perturbation. (C) 1998 Optical Society of America.
引用
收藏
页码:307 / 309
页数:3
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