Chromatin remodeling activities act on UV-damaged nucleosomes and modulate DNA damage accessibility to photolyase

被引：63

作者：

Gaillard, H

Fitzgerald, DJ

Smith, CL

Peterson, CL

Richmond, TJ

Thoma, F ^{[1
]}

机构：

[1] ETH Honggerberg, Inst Zellbiol, CH-8093 Zurich, Switzerland

[2] ETH Honggerberg, Inst Mol Biol, Dept Biol, CH-8093 Zurich, Switzerland

[3] Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA 01605 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2003年 / 278卷 / 20期

关键词：

D O I：

10.1074/jbc.M300770200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleosomes inhibit DNA repair in vitro, suggesting that chromatin remodeling activities might be required for efficient repair in vivo. To investigate how structural and dynamic properties of nucleosomes affect damage recognition and processing, we investigated repair of UV lesions by photolyase on a nucleosome positioned at one end of a 226-bp-long DNA fragment. Repair was slow in the nucleosome but efficient outside. No disruption or movement of the nucleosome was observed after UV irradiation and during repair. However, incubation with the nucleosome remodeling complex SWI/SNF and ATP altered the conformation of nucleosomal DNA as judged by UV photo-footprinting and promoted more homogeneous repair. Incubation with yISW2 and ATP moved the nucleosome to a more central position, thereby altering the repair pattern. This is the first demonstration that two different chromatin remodeling complexes can act on UV-damaged nucleosomes and modulate repair. Similar activities might relieve the inhibitory effect of nucleosomes on DNA repair processes in living cells.

引用

页码：17655 / 17663

页数：9

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