Apertureless scanning near-field fluorescence microscopy in liquids

被引:13
作者
Fragola, A
Aigouy, L
Mignotte, PY
Formanek, F
De Wilde, Y
机构
[1] ESPCI, CNRS, UPR A0005, Lab Spectroscopie Lumiere Polarisee, F-75231 Paris 5, France
[2] Univ Paris 06, F-75252 Paris 5, France
关键词
scanning microscopy; fluorescence; near-field optical microscopy;
D O I
10.1016/j.ultramic.2004.04.002
中图分类号
TH742 [显微镜];
学科分类号
摘要
We show that apertureless scanning near-field optical microscopes that use sharp vibrating conical tips can be operated in liquid environments. We have investigated the damping of the tip oscillation as a function of its shape and as a function of its depth under the liquid surface. The degradation of the quality factor from 150 in air down to 15 in liquid does not impede to perform topographic and optical measurements with a very good sensitivity. As an example of application, we present near-field fluorescence images of dye-doped polystyrene spheres immersed in a liquid. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:47 / 54
页数:8
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