Two potato proteins, including a novel RING finger protein (HIP1), interact with the potyviral multifunctional protein HCpro

被引：41

作者：

Guo, DY

Spetz, C

Saarma, M

Valkonen, JPT

机构：

[1] Univ Helsinki, Inst Biotechnol, Viikki Bioctr, FIN-00014 Helsinki, Finland

[2] SLU, Genet Ctr, Dept Plant Biol, SE-75007 Uppsala, Sweden

[3] Univ Helsinki, Dept Appl Biol, FIN-00014 Helsinki, Finland

[4] Wuhan Univ, Coll Life Sci, Virol Ctr, Wuhan 430072, Peoples R China

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 2003年 / 16卷 / 05期

关键词：

D O I：

10.1094/MPMI.2003.16.5.405

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Potyviral helper-component proteinase (HCpro) is a multifunctional protein exerting its cellular functions in interaction with putative host proteins. In this study, cellular protein partners of the HCpro encoded by Potato virus A (PVA) (genus Potyvirus) were screened in a potato leaf cDNA library using a yeast two-hybrid system. Two cellular proteins were obtained that interact specifically with PVA HCpro in yeast and in the two in vitro binding assays used. Both proteins are encoded by single-copy genes in the potato genome. Analysis of the deduced amino acid sequences revealed that one (HIP1) of the two HCpro interactors is a novel RING finger protein. The sequence of the other protein (HIP2) showed no resemblance to the protein sequences available from databanks and has known biological functions.

引用

页码：405 / 410

页数：6

共 42 条

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