Characterization of the Insoluble Proteome of Lactococcus lactis by SDS-PAGE LC-MS/MS Leads to the Identification of New Markers of Adaptation of the Bacteria to the Mouse Digestive Tract

被引：24

作者：

Beganovic, Jasna

Guillot, Alain

van de Guchte, Maarten ^{[2
]}

Jouan, Anne ^{[3
]}

Gitton, Christophe ^{[4
]}

Loux, Valentin ^{[5
]}

Roy, Karine ^{[4
]}

Huet, Sylvie ^{[3
]}

Monod, Herve ^{[3
]}

Monnet, Veronique ^{[1
,4
]}

机构：

[1] INRA, Unite Biochim Bacterienne, PAPPSO, F-78352 Jouy En Josas, France

[2] INRA, Genet Microbienne UR895, F-78352 Jouy En Josas, France

[3] INRA, Math & Informat Appl UR341, F-78352 Jouy En Josas, France

[4] INRA, Biochim Bacterienne UR477, F-78352 Jouy En Josas, France

[5] INRA, Math UR1077, F-78352 Jouy En Josas, France

来源：

JOURNAL OF PROTEOME RESEARCH | 2010年 / 9卷 / 02期

关键词：

Lactococcus lactis; cell envelope; proteome; protein abundance; comparative proteomics; LC-MS/MS; digestive tract; spectral counting; peptide counting; membrane protein; ELONGATION-FACTOR TU; MASS-SPECTROMETRY; MEMBRANE-PROTEINS; BACILLUS-SUBTILIS; CELL-ENVELOPE; QUANTITATIVE PROTEOMICS; CANDIDATE PROTEINS; ABSOLUTE PROTEIN; GENE-EXPRESSION; ABUNDANCE;

D O I：

10.1021/pr9000866

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We characterized the insoluble proteome of Lactococcus lactis using 1D electrophoresis-LC-MS/MS and identified 313 proteins with at least two different peptides. The identified proteins include 89 proteins having a predicted signal peptide and 25 predicted to be membrane-located. In addition, 67 proteins had alkaline isoelectric point values. Using spectra and peptide counts, we compared protein abundances in two different conditions: growth in rich medium, and after transit in the mouse digestive tract. We identified the large mechanosensitive channel and a putative cation transporter as membrane markers of bacterial adaptation to the digestive tract.

引用

页码：677 / 688

页数：12

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[51] An improved strategy for selective and efficient enrichment of integral plasma membrane proteins of mycobacteria [J].