Rapid hyperspectral fluorescence lifetime imaging

被引：41

作者：

De Beule, Pieter ^{[1
]}

Owen, Dylan M. ^{[1
]}

Manning, Hugh B. ^{[1
]}

Talbot, Clifford B. ^{[1
]}

Requejo-Isidro, Jose ^{[1
]}

Dunsby, Christopher ^{[1
]}

McGinty, James ^{[1
]}

Benninger, Richard K. P. ^{[1
]}

Elson, Daniel S. ^{[1
]}

Munro, Ian ^{[1
]}

Lever, M. John ^{[1
]}

Anand, Praveen ^{[1
]}

Neil, Mark A. A. ^{[1
]}

French, Paul M. W. ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Phys, Photon Grp, London SW7 2AZ, England

来源：

MICROSCOPY RESEARCH AND TECHNIQUE | 2007年 / 70卷 / 05期

关键词：

fluorescence lifetime imaging; FLIM; hyperspectral imaging; tissue autofluorescence;

D O I：

10.1002/jemt.20434

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

We report a rapid hyperspectral fluorescence lifetime imaging (FLIM) instrument that exploits high-speed FLIM technology in a line-scanning microscope. We demonstrate the acquisition of whole-field optically sectioned hyperspectral fluorescence lifetime image stacks (with 32 spectral bins) in less than 40 s and illustrate its application to unstained biological tissue.

引用

页码：481 / 484

页数：4

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