Proteins modified with DNAzymes or aptamers act as biosensors or biosensor labels

被引：100

作者：

Shlyahovsky, Bella ^{[1
]}

Li, Di ^{[1
]}

Katz, Eugenii ^{[1
]}

Willner, Itamar ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Inst Chem, IL-91904 Jerusalem, Israel

来源：

BIOSENSORS & BIOELECTRONICS | 2007年 / 22卷 / 11期

关键词：

DNAzyme; aptamer; glucose oxidase; thrombin; biosensor; chemiluminescence;

D O I：

10.1016/j.bios.2006.10.009

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Hybrid systems composed of a glucose oxidase (GOx)/peroxidase-mimicking DNAzyme, and of microperoxidase-11 (MP-11)/anti-thrombin aptamer were synthesized. The hybrid systems were employed as amplifying labels for the colorimetric or chemiluminescence detection of an enzyme functions, and thrombin analysis, respectively. In the GOx/DNAzyme system, the GOx-mediated oxidation of glucose led to the formation of H2O2, and this activated the oxidation of ABTS to a colored product, or to the generation of chemiluminescence in the presence of luminol. The MP-11/anti-thrombin aptamer enabled the amplified analysis of thrombin by the MP-11-mediated generation of chemiluminescence in the presence of luminol/H2O2. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：2570 / 2576

页数：7

共 41 条

[1]

ADAMS PA, 1991, PEROXIDASES CHEM BIO, V2, P171

[2] Elementary arithmetic operations by enzymes: A model for metabolic pathway based computing [J].

Baron, R ;

Lioubashevski, O ;

Katz, E ;

Niazov, T ;

Willner, I .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (10) :1572-1576

[3] Engineered allosteric ribozymes as biosensor components [J].

Breaker, RR .

CURRENT OPINION IN BIOTECHNOLOGY, 2002, 13 (01) :31-39

[4] SYNTHETIC RIBOZYMES AND THE FIRST DEOXYRIBOZYME [J].

BURGSTALLER, P ;

FAMULOK, M .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1995, 34 (11) :1189-1192

[5] Magnetic bead-based chemiluminescence detection of sequence-specific DNA by using catalytic nucleic acid labels [J].

Cao, ZJ ;

Li, ZX ;

Zhao, YJ ;

Song, YM ;

Lu, JZ .

ANALYTICA CHIMICA ACTA, 2006, 557 (1-2) :152-158

[6] INVITRO SELECTION OF RNA MOLECULES THAT BIND SPECIFIC LIGANDS [J].

ELLINGTON, AD ;

SZOSTAK, JW .

NATURE, 1990, 346 (6287) :818-822

[7] INVITRO SELECTION OF SPECIFIC LIGAND-BINDING NUCLEIC-ACIDS [J].

FAMULOK, M ;

SZOSTAK, JW .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1992, 31 (08) :979-988

[8]

FAMULOK M, 1993, NUCLEIC ACIDS MOL BI, V7, P271

[9] Pt nanopartictes functionalized with nucleic acid act as catalytic labels for the chemiluminescent detection of DNA and proteins [J].

Gill, Ron ;

Polsky, Ronen ;

Willner, Itamar .

SMALL, 2006, 2 (8-9) :1037-1041

[10] Aptamer beacons for the direct detection of proteins [J].

Hamaguchi, N ;

Ellington, A ;

Stanton, M .

ANALYTICAL BIOCHEMISTRY, 2001, 294 (02) :126-131

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