Proteins modified with DNAzymes or aptamers act as biosensors or biosensor labels

被引：100

作者：

Shlyahovsky, Bella ^{[1
]}

Li, Di ^{[1
]}

Katz, Eugenii ^{[1
]}

Willner, Itamar ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Inst Chem, IL-91904 Jerusalem, Israel

来源：

BIOSENSORS & BIOELECTRONICS | 2007年 / 22卷 / 11期

关键词：

DNAzyme; aptamer; glucose oxidase; thrombin; biosensor; chemiluminescence;

D O I：

10.1016/j.bios.2006.10.009

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Hybrid systems composed of a glucose oxidase (GOx)/peroxidase-mimicking DNAzyme, and of microperoxidase-11 (MP-11)/anti-thrombin aptamer were synthesized. The hybrid systems were employed as amplifying labels for the colorimetric or chemiluminescence detection of an enzyme functions, and thrombin analysis, respectively. In the GOx/DNAzyme system, the GOx-mediated oxidation of glucose led to the formation of H2O2, and this activated the oxidation of ABTS to a colored product, or to the generation of chemiluminescence in the presence of luminol. The MP-11/anti-thrombin aptamer enabled the amplified analysis of thrombin by the MP-11-mediated generation of chemiluminescence in the presence of luminol/H2O2. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：2570 / 2576

页数：7