A reagentless signal-on architecture for electronic, aptamer-based sensors via target-induced strand displacement

被引：472

作者：

Xiao, Y

Piorek, BD

Plaxco, KW ^{[1
]}

Heeger, AJ

机构：

[1] Univ Calif Santa Barbara, Program BioMol Sci & Engn, Dept Phys, Santa Barbara, CA 93106 USA

[2] Univ Calif Santa Barbara, Program BioMol Sci & Engn, Dept Mat, Santa Barbara, CA 93106 USA

[3] Univ Calif Santa Barbara, Program BioMol Sci & Engn, Inst Polymers & Organ Solids, Santa Barbara, CA 93106 USA

[4] Univ Calif Santa Barbara, Dept Chem & Biochem, Santa Barbara, CA 93106 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 51期

关键词：

D O I：

10.1021/ja056555h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Thrombin binding stabilizes the alternative G-quadruplex conformation of the aptamer, liberating the methylene blue (MB)-tagged oligonucleotide to produce a flexible, single-stranded DNA element. This allows the MB tag to collide with the gold electrode surface, producing a readily detectable Faradaic current at thrombin concentrations as low as ∼3 nM. Copyright © 2005 American Chemical Society.

引用

页码：17990 / 17991

页数：2

共 22 条

[1] SELECTION OF SINGLE-STRANDED-DNA MOLECULES THAT BIND AND INHIBIT HUMAN THROMBIN
BOCK, LC
GRIFFIN, LC
LATHAM, JA
VERMAAS, EH
TOOLE, JJ
[J]. NATURE, 1992, 355 (6360) : 564 - 566
[2] Proximity extension of circular DNA aptamers with real-time protein detection
Di Giusto, DA
Wlassoff, WA
Gooding, JJ
Messerle, BA
King, GC
[J]. NUCLEIC ACIDS RESEARCH, 2005, 33 (06) : 1 - 7
[3] A DNA-based machine that can cyclically bind and release thrombin
Dittmer, WU
Reuter, A
Simmel, FC
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (27) : 3550 - 3553
[4] INVITRO SELECTION OF RNA MOLECULES THAT BIND SPECIFIC LIGANDS
ELLINGTON, AD
SZOSTAK, JW
[J]. NATURE, 1990, 346 (6287) : 818 - 822
[5] Electrochemical interrogation of conformational changes as a reagentless method for the sequence-specific detection of DNA
Fan, CH
Plaxco, KW
Heeger, AJ
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (16) : 9134 - 9137
[6] Synthetic DNA aptamers to detect protein molecular variants in a high-throughput fluorescence quenching assay
Fang, XH
Sen, A
Vicens, M
Tan, WH
[J]. CHEMBIOCHEM, 2003, 4 (09) : 829 - 834
[7] In vitro selection and evaluation of RNA aptamers that recognize arginine-rich-motif model peptide on a quartz-crystal microbalance
Fukusho, S
Furusawa, H
Okahata, Y
[J]. CHEMICAL COMMUNICATIONS, 2002, (01) : 88 - 89
[8] Aptamer beacons for the direct detection of proteins
Hamaguchi, N
Ellington, A
Stanton, M
[J]. ANALYTICAL BIOCHEMISTRY, 2001, 294 (02) : 126 - 131
[9] Optical sensors based on hybrid aptamer/conjugated polymer complexes
Ho, HA
Leclerc, M
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (05) : 1384 - 1387
[10] High-affinity RNA as a recognition element in a biosensor
Kleinjung, F
Klussmann, S
Erdmann, VA
Scheller, FW
Fürste, JP
Bier, FF
[J]. ANALYTICAL CHEMISTRY, 1998, 70 (02) : 328 - 331

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