A reagentless signal-on architecture for electronic, aptamer-based sensors via target-induced strand displacement

被引:472
作者
Xiao, Y
Piorek, BD
Plaxco, KW [1 ]
Heeger, AJ
机构
[1] Univ Calif Santa Barbara, Program BioMol Sci & Engn, Dept Phys, Santa Barbara, CA 93106 USA
[2] Univ Calif Santa Barbara, Program BioMol Sci & Engn, Dept Mat, Santa Barbara, CA 93106 USA
[3] Univ Calif Santa Barbara, Program BioMol Sci & Engn, Inst Polymers & Organ Solids, Santa Barbara, CA 93106 USA
[4] Univ Calif Santa Barbara, Dept Chem & Biochem, Santa Barbara, CA 93106 USA
关键词
D O I
10.1021/ja056555h
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Thrombin binding stabilizes the alternative G-quadruplex conformation of the aptamer, liberating the methylene blue (MB)-tagged oligonucleotide to produce a flexible, single-stranded DNA element. This allows the MB tag to collide with the gold electrode surface, producing a readily detectable Faradaic current at thrombin concentrations as low as ∼3 nM. Copyright © 2005 American Chemical Society.
引用
收藏
页码:17990 / 17991
页数:2
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