A reagentless signal-on architecture for electronic, aptamer-based sensors via target-induced strand displacement

被引：472

作者：

Xiao, Y

Piorek, BD

Plaxco, KW ^{[1
]}

Heeger, AJ

机构：

[1] Univ Calif Santa Barbara, Program BioMol Sci & Engn, Dept Phys, Santa Barbara, CA 93106 USA

[2] Univ Calif Santa Barbara, Program BioMol Sci & Engn, Dept Mat, Santa Barbara, CA 93106 USA

[3] Univ Calif Santa Barbara, Program BioMol Sci & Engn, Inst Polymers & Organ Solids, Santa Barbara, CA 93106 USA

[4] Univ Calif Santa Barbara, Dept Chem & Biochem, Santa Barbara, CA 93106 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 51期

关键词：

D O I：

10.1021/ja056555h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Thrombin binding stabilizes the alternative G-quadruplex conformation of the aptamer, liberating the methylene blue (MB)-tagged oligonucleotide to produce a flexible, single-stranded DNA element. This allows the MB tag to collide with the gold electrode surface, producing a readily detectable Faradaic current at thrombin concentrations as low as ∼3 nM. Copyright © 2005 American Chemical Society.

引用

页码：17990 / 17991

页数：2

共 22 条

[11] A fiber-optic microarray biosensor using aptamers as receptors
Lee, M
Walt, DR
[J]. ANALYTICAL BIOCHEMISTRY, 2000, 282 (01) : 142 - 146
[12] New trends in affinity sensing: aptamers for ligand binding
Luzi, E
Minunni, M
Tombelli, S
Mascini, M
[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2003, 22 (11) : 810 - 818
[13] STRUCTURAL AND FUNCTIONAL-CHARACTERIZATION OF POTENT ANTITHROMBOTIC OLIGONUCLEOTIDES POSSESSING BOTH QUADRUPLEX AND DUPLEX MOTIFS
MACAYA, RF
WALDRON, JA
BEUTEL, BA
GAO, HT
JOESTEN, ME
YANG, MH
PATEL, R
BERTELSEN, AH
COOK, AF
[J]. BIOCHEMISTRY, 1995, 34 (13) : 4478 - 4492
[14] An ambiguous structure of a DNA 15-mer thrombin complex
Padmanabhan, K
Tulinsky, A
[J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 1996, 52 : 272 - 282
[15] Aptamer-functionalized Au nanoparticles for the amplified optical detection of thrombin
Pavlov, V
Xiao, Y
Shlyahovsky, B
Willner, I
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (38) : 11768 - 11769
[16] Adapting selected nucleic acid ligands (aptamers) to biosensors
Potyrailo, RA
Conrad, RC
Ellington, AD
Hieftje, GM
[J]. ANALYTICAL CHEMISTRY, 1998, 70 (16) : 3419 - 3425
[17] SELECTION INVITRO OF AN RNA ENZYME THAT SPECIFICALLY CLEAVES SINGLE-STRANDED-DNA
ROBERTSON, DL
JOYCE, GF
[J]. NATURE, 1990, 344 (6265) : 467 - 468
[18] Micromechanical detection of proteins using aptamer-based receptor molecules
Savran, CA
Knudsen, SM
Ellington, AD
Manalis, SR
[J]. ANALYTICAL CHEMISTRY, 2004, 76 (11) : 3194 - 3198
[19] Aptamer-based folding fluorescent sensor for cocaine
Stojanovic, MN
de Prada, P
Landry, DW
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (21) : 4928 - 4931
[20] SYSTEMATIC EVOLUTION OF LIGANDS BY EXPONENTIAL ENRICHMENT - RNA LIGANDS TO BACTERIOPHAGE-T4 DNA-POLYMERASE
TUERK, C
GOLD, L
[J]. SCIENCE, 1990, 249 (4968) : 505 - 510

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