High-affinity RNA as a recognition element in a biosensor

被引：86

作者：

Kleinjung, F

Klussmann, S

Erdmann, VA

Scheller, FW

Fürste, JP

Bier, FF

机构：

[1] Univ Potsdam, Max Delbruck Ctr Mol Med, Inst Biochem & Mol Physiol, D-13125 Berlin, Germany

[2] Free Univ Berlin, Inst Biochem, D-14195 Berlin, Germany

来源：

ANALYTICAL CHEMISTRY | 1998年 / 70卷 / 02期

关键词：

D O I：

10.1021/ac9706483

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A biosensor for L-adenosine employing high-affinity RNA as binder is reported. Real-time measurement is obtained using total internal reflection fluorescence in a fiber-optic format. High-affinity RNA was attached to the core of a multimode fiber via and avidin-biotin bridge. The sensor measures binding of FITC-labeled L-adenosine. The interaction was fully characterized by the determination of association and dissociation rates: k(D) = 0.0119 s(-1); k(A) = 2200 M-1 s(-1). Competitive inhibition with L-adenosine enables this device to detect L-adenosine in the submicromolar range.

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收藏

页码：328 / 331

页数：4

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