The chemical composition of animal cells and their intracellular compartments reconstructed from 3D mass spectrometry

被引：85

作者：

Breitenstein, Daniel

Rommel, Christina E.

Moellers, Rudolf

Wegener, Joachim

Hagenhoff, Birgit

机构：

[1] Univ Munster, Inst Biochem, D-48149 Munster, Germany

[2] Tascon GmbH, D-48149 Munster, Germany

[3] IonTof GmbH, D-48149 Munster, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 28期

关键词：

analytical methods; cell imaging; mass spectrometry; surface analysis;

D O I：

10.1002/anie.200604468

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) A peek inside: A C60+ sputter ion source integrated into a time-of-flight secondary-ion mass spectrometer was used to dissect animal cells in a layer-by-layer fashion and analyze the spatial distribution of endogenous molecules. Repeated cycles of sputter erosion and SIMS analysis provided molecular information from the inside of the cells (see picture; red: amino acids, green: phospholipids, blue: glass substrate). © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：5332 / 5335

页数：4

共 19 条

[11]

Holzenburg A., 1998, ELECT MICROSCOPY MIC

[12] Cluster primary ion bombardment of organic materials [J].