Contribution of mononuclear bone marrow cells to carbon tetrachloride-induced liver fibrosis in rats

被引:10
作者
Cao, Bao-Qiang
Lin, Ji-Zong
Zhong, Yue-Si
Huang, Shao-Bin
Lin, Nan
Tang, Zhao-Feng
Chen, Rui
Xiang, Peng
Xu, Rui-Yun [1 ]
机构
[1] Sun Yat Sen Univ, Third Affiliated Hosp, Dept Hepatobil Surg, Guangzhou 510630, Guangdong, Peoples R China
[2] Sun Yat Sen Univ, Ctr Stem Cell Biol & Tissue Engn, Guangzhou 510080, Guangdong, Peoples R China
关键词
bone marrow cell; transplantation; liver cirrhosis; rat model;
D O I
10.3748/wjg.v13.i12.1851
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
AIM: To study the inhibitory effect of mononuclear bone marrow cell (BMC) transplantation on carbon tetrachloride (CCl4)-induced liver fibrosis in rats. METHODS: Rat liver fibrosis models were induced by CCl4 and alcohol administration. After 8 wk, twenty rats were randomly allocated into treatment group (n = 10) and control group (n = 10). BMC were infused into the rats in treatment group via the portal vein, while heparinized saline was infused in control group. CCl4 was hypodermically injected into the rats twice a week for 4 wk. At the end of wk 12, all rats were humanely sacrificed. Liver samples were taken and stained with HE or Masson trichrome. The general conditions, liver fibrosis (hydroxyproline and collagen fibre) and liver pathological grades in rats were evaluated. RESULTS: The general conditions of the rats in treatment group improved markedly, but not in control group. Hydroxyproline was 504.6 +/- 128.8 mu g/g in treatment group, and 596.0 +/- 341.8 mu g/g in control group. The percentage of collagen fibre was 3.75% +/- 0.98% in treatment group and 5.02% +/- 0.44% in control group. There was a significant difference between the two groups (P < 0.05). Liver pathological grade decreased from grade IV to grade III partially in treatment group (P < 0.05) with no obvious improvement in control group (P > 0.05). There was a significant difference between treatment group and control group (P < 0.05). CONCLUSION: Transplantation of BMC can improve liver fibrosis due to chronic liver injury in rats. (C) 2007 The WJG Press. All rights reserved.
引用
收藏
页码:1851 / 1856
页数:6
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