Investigation of energy transfer between CdTe nanocrystals on polystyrene beads and dye molecules for FRET-SNOM applications

被引:39
作者
Müller, F
Götzinger, S
Gaponik, N
Weller, H
Mlynek, J
Benson, O
机构
[1] Humboldt Univ, Inst Phys, D-10117 Berlin, Germany
[2] Univ Hamburg, Inst Chem Phys, D-20146 Hamburg, Germany
关键词
D O I
10.1021/jp049183s
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
We report results on the observation of energy transfer between CdTe nanocrystals and AlexaFluor dye molecules. The nanocrystals were attached to the surface of polystyrene latex beads of 450 nm in diameter by means of the layer-by-layer technique. Energy transfer was observed as signal enhancement in confocal imaging of a single bead covered with an AlexaFluor film. This was verified by detecting quenching of the donor emission and enhancement of the acceptor emission in spectral measurements. A fluorescence-resonance energy transfer scanning near-field optical microscopy (FRET-SNOM) tip was made from a single bead coated with CdTe attached to a near-field tip. We report first results on FRET-SNOM imaging with this tip.
引用
收藏
页码:14527 / 14534
页数:8
相关论文
共 45 条
[21]   A single gold particle as a probe for apertureless scanning near-field optical microscopy [J].
Kalkbrenner, T ;
Ramstein, M ;
Mlynek, J ;
Sandoghdar, V .
JOURNAL OF MICROSCOPY-OXFORD, 2001, 202 :72-76
[22]  
Lakowicz J. R., 2006, Principles of Fluorescence Spectroscopy Springer
[23]   DEVELOPMENT OF A 500-A SPATIAL-RESOLUTION LIGHT-MICROSCOPE .1. LIGHT IS EFFICIENTLY TRANSMITTED THROUGH GAMMA-16 DIAMETER APERTURES [J].
LEWIS, A ;
ISAACSON, M ;
HAROOTUNIAN, A ;
MURAY, A .
ULTRAMICROSCOPY, 1984, 13 (03) :227-231
[24]   A LIGHT-SOURCE SMALLER THAN THE OPTICAL WAVELENGTH [J].
LIEBERMAN, K ;
HARUSH, S ;
LEWIS, A ;
KOPELMAN, R .
SCIENCE, 1990, 247 (4938) :59-61
[25]  
Mattoussi H., 2002, OPTICAL BIOSENSORS P, P537
[26]   Self-assembled nanoscale biosensors based on quantum dot FRET donors [J].
Medintz, IL ;
Clapp, AR ;
Mattoussi, H ;
Goldman, ER ;
Fisher, B ;
Mauro, JM .
NATURE MATERIALS, 2003, 2 (09) :630-638
[27]   Optical microscopy using a single-molecule light source [J].
Michaelis, J ;
Hettich, C ;
Mlynek, J ;
Sandoghdar, V .
NATURE, 2000, 405 (6784) :325-328
[28]   SYNTHESIS AND CHARACTERIZATION OF NEARLY MONODISPERSE CDE (E = S, SE, TE) SEMICONDUCTOR NANOCRYSTALLITES [J].
MURRAY, CB ;
NORRIS, DJ ;
BAWENDI, MG .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (19) :8706-8715
[29]   Correlation between fluorescence intermittency and spectral diffusion in single semiconductor quantum dots [J].
Neuhauser, RG ;
Shimizu, KT ;
Woo, WK ;
Empedocles, SA ;
Bawendi, MG .
PHYSICAL REVIEW LETTERS, 2000, 85 (15) :3301-3304
[30]   Near-field optical imaging using metal tips illuminated by higher-order Hermite-Gaussian beams [J].
Novotny, L ;
Sánchez, EJ ;
Xie, XS .
ULTRAMICROSCOPY, 1998, 71 (1-4) :21-29