Ischemic tolerance affects the adenylation state of GluR2 mRNA

被引：7

作者：

Alsbo, CW ^{[1
]}

Wrang, ML ^{[1
]}

Nielsen, M ^{[1
]}

Diemer, NH ^{[1
]}

机构：

[1] Univ Copenhagen, Inst Mol Pathol, Neuropathol Lab, Pharmabiotec Res Ctr, DK-2100 Copenhagen, Denmark

来源：

NEUROREPORT | 2000年 / 11卷 / 14期

关键词：

adenylation; AMPA; brain; global ischemia; in situ hybridization; ischemia; quantitative PCR; rat; tolerance;

D O I：

10.1097/00001756-200009280-00046

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

To analyse GluR2 regulations in the rat hippocampal CAI region following global and tolerance-inducing ischemia in situ hybridization (ISH) and quantitative PCR (Q-PCR) was applied. In addition, cDNA was synthesised from two different primer combinations in order to elucidate possible differences in the adenylation state of GluR2 mRNA. Following global ischemia, ISH and Q-PCR both showed reductions to half of control levels of GluR2 mRNA in consent with previously published results. Following tolerance induction, ISH showed no changes, whereas PCR analysis showed up-regulation to 228% of control value for the general cDNA synthesis, and no change for the specific cDNA synthesis. This indicates that tolerance-inducing ischemia does not increase the amount of GluR2 mRNA; instead polyadenylation of the existing GluR2 mRNA pool takes place. NeuroReport 11:3279-3282 (C) 2000 Lippincott Williams & Wilkins.

引用

页码：3279 / 3282

页数：4

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