Selective immobilization of DNA and antibody probes on electrode arrays: Simultaneous electrochemical detection of DNA and protein on a single platform

被引：55

作者：

Harper, Jason C. ^{[1
]}

Polsky, Ronen ^{[1
]}

Wheeler, David R. ^{[1
]}

Dirk, Shawn M. ^{[1
]}

Brozik, Susan M. ^{[1
]}

机构：

[1] Sandia Natl Labs, Albuquerque, NM 87185 USA

来源：

LANGMUIR | 2007年 / 23卷 / 16期

关键词：

D O I：

10.1021/la701775g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A proof of concept procedure for the electroaddressable covalent immobilization of DNA and protein on arrayed electrodes along with simultaneous detection of multiple bioagents in the same sample solution is described. Carboxyphenyldiazonium was selectively deposited onto five of nine individually addressable electrodes in an array via bias assisted assembly. Amine functionalized DNA probes were covalently coupled to the carboxyl surface via carbodiimide chemistry. This was followed by the covalent immobilization of diazonium-antibody conjugates into the remaining four electrodes via cyclic voltammetry. Simultaneous electrochemical detection of a DNA sequence related to the breast cancer BRCA1 gene and the human cytokine protein interleukin-12, which is a substantial component in the immune system response and attack of tumor cells, is reported. These results demonstrate the possibility of selective patterning of diverse biomolecules on a single device and may have significant implications for future development of microarrays and biosensors.

引用

页码：8285 / 8287

页数：3

共 20 条

[11] Electrically addressable biomolecular functionalization of carbon nanotube and carbon nanofiber electrodes [J].