Transcription of homeobox-containing genes detected in cDNA libraries derived from human unfertilized oocytes and preimplantation embryos

被引:25
作者
Adjaye, J
Monk, M
机构
[1] Inst Child Hlth, Mol Med Unit, London WC1N 1EH, England
[2] Inst Child Hlth, Mol Embryol Unit, London WC1N 1EH, England
关键词
embryonic cDNA libraries; homeobox gene expression; human preimplantation development; maternal mRNA; OCT-4;
D O I
10.1093/molehr/6.8.707
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Genes containing the evolutionarily conserved homeodomain sequence encode a family of DNA-binding transcription factors whose functions are crucial for embryonic development in vertebrates, invertebrates and plants. We describe the detection and analysis of transcripts of homeobox-containing genes present in cDNA libraries generated from human unfertilized oocytes, single cleavage stage embryos (2-cell, 4-cell, 8-cell and blastocyst) and a 10-week old whole fetus. Using degenerate primers derived from sequences within helix 1 and helix 3 of the highly conserved region of the Antennapedia-class homeodomain, a 166 bp band was detected in all the cDNA libraries tested. Subcloning of the oocyte-derived band revealed that it contained a heterogeneous group of 166 bp fragments. Sequence analysis of 40 independent clones demonstrated the presence of HOXA7, HOXD8, and HOXD1 sequences, the ubiquitously expressed POU family member, OCT1, and HEX, a homeotic gene expressed in haematopoietic cells.
引用
收藏
页码:707 / 711
页数:5
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