Application of the random amplified polymorphic DNA (RAPD) technique to distinguishing species of the red tide phytoplankton Chattonella (Raphydophyceae)
被引:9
作者:
Murayama-Kayano, E
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机构:Univ Tokyo, Fac Engn, Dept Chem & Biotechnol, Bunkyo Ku, Tokyo 1138656, Japan
Murayama-Kayano, E
Yoshimatsu, S
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机构:Univ Tokyo, Fac Engn, Dept Chem & Biotechnol, Bunkyo Ku, Tokyo 1138656, Japan
Yoshimatsu, S
Kayano, T
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机构:Univ Tokyo, Fac Engn, Dept Chem & Biotechnol, Bunkyo Ku, Tokyo 1138656, Japan
Kayano, T
Nishio, T
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机构:Univ Tokyo, Fac Engn, Dept Chem & Biotechnol, Bunkyo Ku, Tokyo 1138656, Japan
Nishio, T
Ueda, H
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机构:Univ Tokyo, Fac Engn, Dept Chem & Biotechnol, Bunkyo Ku, Tokyo 1138656, Japan
Ueda, H
Nagamune, T
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机构:Univ Tokyo, Fac Engn, Dept Chem & Biotechnol, Bunkyo Ku, Tokyo 1138656, Japan
Nagamune, T
机构:
[1] Univ Tokyo, Fac Engn, Dept Chem & Biotechnol, Bunkyo Ku, Tokyo 1138656, Japan
[2] Akashiwo Res Inst Kagawa Prefecture, Takamatsu, Kagawa 7610111, Japan
[3] Natl Inst Agrobiol Resources, Tsukuba, Ibaraki 3058602, Japan
来源:
JOURNAL OF FERMENTATION AND BIOENGINEERING
|
1998年
/
85卷
/
03期
关键词:
Raphidophyceae;
DNA;
PCR;
RAPD;
red tide;
Chattonella;
D O I:
10.1016/S0922-338X(97)85687-5
中图分类号:
Q81 [生物工程学(生物技术)];
Q93 [微生物学];
学科分类号:
071005 ;
0836 ;
090102 ;
100705 ;
摘要:
The random amplified polymorphic DNA (RAPD) technique was applied to strain and species identification. Four different arbitrary primers were tested with DNAs from seven strains of four species of red tide algae of the genus Chattonella. Two of the primers showed species-dependent profiles, while the other two identified differences between strains. The results indicate that the RAPD technique is useful for distinguishing polymorphisms among species or strains.