OXA-24 Carbapenemase Gene Flanked by XerC/XerD-Like Recombination Sites in Different Plasmids from Different Acinetobacter Species Isolated during a Nosocomial Outbreak

被引：95

作者：

Merino, Maria ^{[1
]}

Acosta, Joshi ^{[2
]}

Poza, Margarita ^{[1
]}

Sanz, Francisca ^{[2
]}

Beceiro, Alejandro ^{[1
]}

Chaves, Fernando ^{[2
]}

Bou, German ^{[1
]}

机构：

[1] Complejo Hosp Univ A Coruna INIBIC, Microbiol Lab, La Coruna 15006, Spain

[2] Hosp 12 Octubre, Microbiol Serv, E-28041 Madrid, Spain

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2010年 / 54卷 / 06期

关键词：

HYDROLYZING OXACILLINASE OXA-58; CLINICAL ISOLATE; BETA-LACTAMASE; BAUMANNII; RESOLUTION; GENOSPECIES-3; RESISTANCE; STABILITY; STRAINS;

D O I：

10.1128/AAC.01674-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A clinical strain of Acinetobacter calcoaceticus resistant to carbapenems was isolated from a blood culture sample from an inpatient in a hospital in Madrid (Spain) during a large outbreak of infection (affecting more than 300 inpatients), caused by a multidrug-resistant Acinetobacter baumannii clone. The carbapenem resistance in both the A. calcoaceticus and A. baumannii clones was due to a bla(OXA-24) gene harbored in different plasmids. The plasmids were fully sequenced, revealing the presence of site-specific recombination binding sites putatively involved in mobilization of the bla(OXA-24) gene. Comparison of plasmids contained in the two strains revealed possible horizontal transmission of resistance genes between the Acinetobacter species.

引用

页码：2724 / 2727

页数：4

共 18 条

[1] Acinetobacter spp, as nosocomial pathogens: Microbiological, clinical, and epidemiological features [J].

BergogneBerezin, E ;

Towner, KJ .

CLINICAL MICROBIOLOGY REVIEWS, 1996, 9 (02) :148-+

[2] OXA-24, a novel class D β-lactamase with carbapenemase activity in an Acinetobacter baumannii clinical strain [J].

Bou, G ;

Oliver, A ;

Martínez-Beltran, J .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (06) :1556-1561

[3] Differences in resolution of mwr-containing plasmid dimers mediated by the Klebsiella pneumoniae and Escherichia coli XerC recombinases:: Potential implications in dissemination of antibiotic resistance genes [J].

Bui, DY ;

Ramiscal, J ;

Trigueros, S ;

Newmark, JS ;

Do, A ;

Sherratt, DJ ;

Tolmasky, ME .

JOURNAL OF BACTERIOLOGY, 2006, 188 (08) :2812-2820

[4] PLASMID PSC101 HARBORS A RECOMBINATION SITE, PSI, WHICH IS ABLE TO RESOLVE PLASMID MULTIMERS AND TO SUBSTITUTE FOR THE ANALOGOUS CHROMOSOMAL ESCHERICHIA-COLI SITE DIF [J].

CORNET, F ;

MORTIER, I ;

PATTE, J ;

LOUARN, JM .

JOURNAL OF BACTERIOLOGY, 1994, 176 (11) :3188-3195

[5] Characterization of pABVA01, a Plasmid Encoding the OXA-24 Carbapenemase from Italian Isolates of Acinetobacter baumannii [J].

D'Andrea, Marco Maria ;

Giani, Tommaso ;

D'Arezzo, Silvia ;

Capone, Alessandro ;

Petrosillo, Nicola ;

Visca, Paolo ;

Luzzaro, Francesco ;

Rossolini, Gian Maria .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2009, 53 (08) :3528-3533

[6] Characterization of the naturally occurring oxacillinase of Acinetobacter baumannii [J].

Héritier, C ;

Poirel, L ;

Fournier, PE ;

Claverie, JM ;

Raoult, D ;

Nordmann, P .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (10) :4174-4179

[7]

Hwa WE, 2009, J MICROBIOL IMMUNOL, V42, P54

[8] Carbapenemase and efflux pump genes in Acinetobacter calcoaceticus-Acinetobacter baumannii complex strains from Singapore [J].

Koh, Tse Hsien ;

Sng, Li-Hwei ;

Wang, Grace Chee Yeng ;

Hsu, Li-Yang ;

Zhao, Yi .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2007, 60 (05) :1173-1174

[9] Modified Hodge and EDTA-disk synergy tests to screen metallo-β-lactamase-producing strains of Pseudomonas and Acinetobacter species [J].

Lee, K ;

Chong, Y ;

Shin, HB ;

Kim, YA ;

Yong, D ;

Yum, JH .

CLINICAL MICROBIOLOGY AND INFECTION, 2001, 7 (02) :88-91

[10] Characterization of the carbapenem-hydrolyzing oxacillinase OXA-58 in an Acinetobacter phenon 6/ct13TU clinical isolate [J].

Marti, Sara ;

Sanchez-Cespedes, Javier ;

Blasco, M. Dolores ;

Espinal, Paula ;

Ruiz, Marc ;

Alba, Veronica ;

Vila, Jordi .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2008, 61 (04) :468-470

← 1 2 →