DNA markers define plastid haplotypes in Arabidopsis thaliana

被引:3
作者
Azhagiri, Arun K. [1 ]
Maliga, Pal [1 ]
机构
[1] Rutgers State Univ, Waksman Inst, Piscataway, NJ 08854 USA
关键词
Arabidopsis thaliana ecotypes; plastid DNA polymorphism; plastid haplotype; plastid genetic marker;
D O I
10.1007/s00294-006-0118-6
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
To identify genetic markers in the Arabidopsis thaliana plastid genome (ptDNA), we amplified and sequenced the rpl2-psbA and rbcL-accD regions in 26 ecotypes. The two regions contained eight polymorphic sites including five insertions and/or deletions (indels) involving changes in the length of A or T mononucleotide repeats and three base substitutions. The 27 alleles defined 15 plastid haplotypes, providing a practical set of ptDNA markers for the Columbia, Landsberg erecta and Wassilewskija ecotypes that are commonly used in genetic studies and also for the C24 and RLD ecotypes that are the most amenable for cell culture manipulations.
引用
收藏
页码:269 / 275
页数:7
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