The outer plate in vertebrate kinetochores is a flexible network with multiple microtubule interactions

被引：101

作者：

Dong, Yimin

VandenBeldt, Kristin J.

Meng, Xing

Khodjakov, Alexey

McEwen, Bruce F. ^{[1
]}

机构：

[1] SUNY Albany, Dept Biomed Sci, Sch Publ Hlth, Albany, NY 12201 USA

[2] New York State Dept Hlth, Wadsworth Ctr, Albany, NY 12201 USA

来源：

NATURE CELL BIOLOGY | 2007年 / 9卷 / 05期

关键词：

D O I：

10.1038/ncb1576

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Intricate interactions between kinetochores and microtubules are essential for the proper distribution of chromosomes during mitosis. A crucial long-standing question is how vertebrate kinetochores generate chromosome motion while maintaining attachments to the dynamic plus ends of the multiple kinetochore MTs (kMTs) in a kinetochore fibre. Here, we demonstrate that individual kMTs in PtK1 cells are attached to the kinetochore outer plate by several fibres that either embed the microtubule plus- end tips in a radial mesh, or extend out from the outer plate to bind microtubule walls. The extended fibres also interact with the walls of nearby microtubules that are not part of the kinetochore fibre. These structural data, in combination with other recent reports, support a network model of kMT attachment wherein the fibrous network in the unbound outer plate, including the Hec1-Ndc80 complex, dissociates and rearranges to form kMT attachments.

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收藏

页码：516 / U56

页数：11

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