The outer plate in vertebrate kinetochores is a flexible network with multiple microtubule interactions

被引：101

作者：

Dong, Yimin

VandenBeldt, Kristin J.

Meng, Xing

Khodjakov, Alexey

McEwen, Bruce F. ^{[1
]}

机构：

[1] SUNY Albany, Dept Biomed Sci, Sch Publ Hlth, Albany, NY 12201 USA

[2] New York State Dept Hlth, Wadsworth Ctr, Albany, NY 12201 USA

来源：

NATURE CELL BIOLOGY | 2007年 / 9卷 / 05期

关键词：

D O I：

10.1038/ncb1576

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Intricate interactions between kinetochores and microtubules are essential for the proper distribution of chromosomes during mitosis. A crucial long-standing question is how vertebrate kinetochores generate chromosome motion while maintaining attachments to the dynamic plus ends of the multiple kinetochore MTs (kMTs) in a kinetochore fibre. Here, we demonstrate that individual kMTs in PtK1 cells are attached to the kinetochore outer plate by several fibres that either embed the microtubule plus- end tips in a radial mesh, or extend out from the outer plate to bind microtubule walls. The extended fibres also interact with the walls of nearby microtubules that are not part of the kinetochore fibre. These structural data, in combination with other recent reports, support a network model of kMT attachment wherein the fibrous network in the unbound outer plate, including the Hec1-Ndc80 complex, dissociates and rearranges to form kMT attachments.

引用

收藏

页码：516 / U56

页数：11

相关论文

共 32 条

[11] Microtubule polymerization dynamics [J].

Desai, A ;

Mitchison, TJ .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1997, 13 :83-117

[12] Measuring the stoichiometry and physical interactions between components elucidates the architecture of the vertebrate kinetochore [J].

Emanuele, MJ ;

McCleland, ML ;

Satinover, DL ;

Stukenberg, PT .

MOLECULAR BIOLOGY OF THE CELL, 2005, 16 (10) :4882-4892

[13] CENP-F is a novel microtubule-binding protein that is essential for kinetochore attachments and affects the duration of the mitotic checkpoint delay [J].

Feng, J. ;

Huang, H. ;

Yen, T. J. .

CHROMOSOMA, 2006, 115 (04) :320-329

[14] Molecular architecture of a kinetochore-microtubule attachment site [J].

Joglekar, Ajit P. ;

Bouck, David C. ;

Molk, Jeffrey N. ;

Bloom, Kerry S. ;

Salmon, Edward D. .

NATURE CELL BIOLOGY, 2006, 8 (06) :581-585

[15] Chromosomes can congress to the metaphase plate before biorientation [J].

Kapoor, TM ;

Lampson, MA ;

Hergert, P ;

Cameron, L ;

Cimini, D ;

Salmon, ED ;

McEwen, BF ;

Khodjakov, A .

SCIENCE, 2006, 311 (5759) :388-391

[16] Chromosome fragments possessing only one kinetochore can congress to the spindle equator [J].

Khodjakov, A ;

Cole, RW ;

McEwen, BF ;

Buttle, KF ;

Rieder, CL .

JOURNAL OF CELL BIOLOGY, 1997, 136 (02) :229-240

[17] The dynamic kinetochore-microtubule interface [J].

Maiato, H ;

DeLuca, J ;

Salmon, ED ;

Earnshaw, WC .

JOURNAL OF CELL SCIENCE, 2004, 117 (23) :5461-5477

[18] A new look at kinetochore structure in vertebrate somatic cells using high-pressure freezing and freeze substitution [J].

McEwen, BF ;

Hsieh, CE ;

Mattheyses, AL ;

Rieder, CL .

CHROMOSOMA, 1998, 107 (6-7) :366-375

[19] Kinetochore fiber maturation in PtK1 cells and its implications for the mechanisms of chromosome congression and anaphase onset [J].

McEwen, BF ;

Heagle, AB ;

Cassels, GO ;

Buttle, KF ;

Rieder, CL .

JOURNAL OF CELL BIOLOGY, 1997, 137 (07) :1567-1580

[20] CENP-E is essential for reliable bioriented spindle attachment, but chromosome alignment can be achieved via redundant mechanisms in mammalian cells [J].

McEwen, BF ;

Chan, GKT ;

Zubrowski, B ;

Savoian, MS ;

Sauer, MT ;

Yen, TJ .

MOLECULAR BIOLOGY OF THE CELL, 2001, 12 (09) :2776-2789

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