Silver nanoparticle labeled immunoresonance scattering spectral assay for trace fibrinogen

被引：14

作者：

Jiang ZhiLiang ^{[1
]}

Chen YuanYuan

Liang AiHui

Tao HuiLin

Tang NingLi

Zhong FuXin

机构：

[1] Guangxi Normal Univ, Sch Environm & Resource, Guilin 541004, Peoples R China

[2] Guangxi Normal Univ, Dept Mat & Chem Engn, Guilin 541004, Peoples R China

来源：

SCIENCE IN CHINA SERIES B-CHEMISTRY | 2007年 / 50卷 / 03期

基金：

中国国家自然科学基金;

关键词：

silver-nanoparticle; fibrinogen; goat anti-human immune fibrinogen serum; resonance scattering spectral assay;

D O I：

10.1007/s11426-007-0064-2

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Silver nanoparticles in size of 8.0 nm was prepared by the trisodium citrate and used to label goat anti-human fibrinogen. In the pH 5.8 Na2HPO4-NaH2O4 buffer solution (PBS) and in the presence of polyethylene glycol (PEG) and KCI, the immune reaction between silver-labeled goat anti-human fibrinogen and fibrinogen took place and led the resonance scattering intensity at 465 nm (I-465) to decreasing. The I-465 decreased intensity was linear to the fibrinogen concentration in the range from 0.067 to 1.67 mu g/mL, with a detection limit of 0.024 mu g/mL. This method was applied to determination of fibrinogen in human plasma, with satisfactory results.

引用

页码：345 / 350

页数：6

共 32 条

[11] Resonance scattering effect of rhodamine dye association nanoparticles and its application to respective determination of trace ClO2 and Cl2 [J].

Jiang, ZL ;

Zhou, SM ;

Liang, AH ;

Kang, CY ;

He, XC .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2006, 40 (13) :4286-4291

[12] Catalytic resonance scattering spectral method for the determination of trace amounts of Se [J].

Jiang, ZL ;

Liu, QY ;

Liu, SP .

TALANTA, 2002, 58 (04) :635-640

[13]

KOEPKE GA, 1980, SCAND J HAEMATOL SUP, V37, P130

[14] Resonance Rayleigh scattering study of the interaction of heparin with some basic diphenyl naphthylmethane dyes [J].

Liu, SP ;

Luo, HQ ;

Li, NB ;

Liu, ZF ;

Zheng, WX .

ANALYTICAL CHEMISTRY, 2001, 73 (16) :3907-3914

[15] Rayleigh light-scattering spectroscopy: Application to the determination of proteins using bromopyrogallol red [J].

Ma, CQ ;

Li, KA ;

Tong, SY .

BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 1997, 70 (01) :129-133

[16]

Magnani B, 1997, AM J CLIN PATHOL, V107, P527

[17]

MAURIZIO C, 2004, ELECTROCHEM COMMUN, V6, P1217

[18]

*NAT COMM CLIN LAB, 1999, H30A PROC DET FIBR P

[19]

NINA L, 2003, EUROP J PHARM BIOPHA, V56, P469

[20]

PALARETI G, 1991, CLIN CHEM, V37, P714

← 1 2 3 4 →