Ex vivo assessment of lymphocyte antioxidant status using the comet assay

被引：84

作者：

Panayiotidis, M

Collins, AR

机构：

[1] Rowett Res Inst, Aberdeen AB21 9SB, Scotland

[2] Boyd Orr Res Ctr, Aberdeen AB21 9SB, Scotland

[3] Univ Aberdeen, Aberdeen AB21 9SB, Scotland

来源：

FREE RADICAL RESEARCH | 1997年 / 27卷 / 05期

关键词：

DNA damage; human lymphocytes; comet assay; dietary antioxidants;

D O I：

10.3109/10715769709065793

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lymphocytes were isolated from volunteers before and after receiving a single supplement of vitamin C, vitamin E or beta-carotene. The lymphocytes were treated with H2O2, and DNA strand breaks were measured by single cell gel electrophoresis (the comet assay). Significant protection against oxidative DNA damage was evident 2-4 h after vitamin C intake, and 18-24 h after consumption of the other antioxidants. Lymphocytes from smokers were more sensitive to DNA damage than those from non-smokers, and they showed at least as great a protective effect with antioxidants.

引用

页码：533 / 537

页数：5

共 9 条

[1] DIETARY CAROTENES, VITAMIN-C, AND VITAMIN-E AS PROTECTIVE ANTIOXIDANTS IN HUMAN CANCERS [J].