Cellular calcium imaging: so, what's new?

被引:32
作者
Brownlee, C [1 ]
机构
[1] Marine Biol Assoc United Kingdom Lab, Plymouth PL1 2PB, Devon, England
关键词
D O I
10.1016/S0962-8924(00)01799-2
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The ability to uncover the fine details of intracellular Ca2+ signals has improved remarkably in recent years, largely as a result of developments in methods for reporting Ca2+, coupled with great improvements in measurement instrumentation. The cell biologist wishing to image intracellular Ca2+ has a range of options to consider. These include the use of photoproteins, commercially available fluorescent indicators or the Mar generation of fluorescent protein Ca2+ probes. Molecular biology and biophysics are Mow joining forces to bring major advances in the art of deciphering the complexity of spatiotemporal Ca2+ signals.
引用
收藏
页码:451 / 457
页数:7
相关论文
共 47 条
[41]  
2-6
[42]   POSSIBILITY OF SIMULTANEOUSLY MEASURING LOW AND HIGH-CALCIUM CONCENTRATIONS USING FURA-2 AND LIFETIME-BASED SENSING [J].
SZMACINSKI, H ;
LAKOWICZ, JR .
CELL CALCIUM, 1995, 18 (01) :64-75
[43]   Measurement of intracellular calcium [J].
Takahashi, A ;
Camacho, P ;
Lechleiter, JD ;
Herman, B .
PHYSIOLOGICAL REVIEWS, 1999, 79 (04) :1089-1125
[44]   Modulation by K+ channels of action potential-evoked intracellular Ca2+ concentration rises in rat cerebellar basket cell axons [J].
Tan, YP ;
Llano, I .
JOURNAL OF PHYSIOLOGY-LONDON, 1999, 520 (01) :65-78
[45]   Microscopic properties of elementary Ca2+ release sites in nonexcitable cells [J].
Thomas, D ;
Lipp, P ;
Tovey, SC ;
Berridge, MJ ;
Li, WH ;
Tsien, RY ;
Bootman, MD .
CURRENT BIOLOGY, 2000, 10 (01) :8-15
[46]   Distinct calcium signaling pathways regulate calmodulin gene expression in tobacco [J].
van der Luit, AH ;
Olivari, C ;
Haley, A ;
Knight, MR ;
Trewavas, AJ .
PLANT PHYSIOLOGY, 1999, 121 (03) :705-714
[47]   Turning of nerve growth cones induced by localized increases in intracellular calcium ions [J].
Zheng, JQ .
NATURE, 2000, 403 (6765) :89-93