Cellular calcium imaging: so, what's new?

被引:32
作者
Brownlee, C [1 ]
机构
[1] Marine Biol Assoc United Kingdom Lab, Plymouth PL1 2PB, Devon, England
关键词
D O I
10.1016/S0962-8924(00)01799-2
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The ability to uncover the fine details of intracellular Ca2+ signals has improved remarkably in recent years, largely as a result of developments in methods for reporting Ca2+, coupled with great improvements in measurement instrumentation. The cell biologist wishing to image intracellular Ca2+ has a range of options to consider. These include the use of photoproteins, commercially available fluorescent indicators or the Mar generation of fluorescent protein Ca2+ probes. Molecular biology and biophysics are Mow joining forces to bring major advances in the art of deciphering the complexity of spatiotemporal Ca2+ signals.
引用
收藏
页码:451 / 457
页数:7
相关论文
共 47 条
[21]   CIRCADIAN OSCILLATIONS OF CYTOSOLIC AND CHLOROPLASTIC FREE CALCIUM IN PLANTS [J].
JOHNSON, CH ;
KNIGHT, MR ;
KONDO, T ;
MASSON, P ;
SEDBROOK, J ;
HALEY, A ;
TREWAVAS, A .
SCIENCE, 1995, 269 (5232) :1863-1865
[22]   Ca2+ fluorescence imaging with pico- and femtosecond two-photon excitation:: Signal and photodamage [J].
Koester, HJ ;
Baur, D ;
Uhl, R ;
Hell, SW .
BIOPHYSICAL JOURNAL, 1999, 77 (04) :2226-2236
[23]  
LAMBERT D, 1999, METHODS MOL BIOL, V114
[24]   Supralinear Ca2+ signaling by cooperative and mobile Ca2+ buffering in Purkinje neurons [J].
Maeda, H ;
Ellis-Davies, GCR ;
Ito, K ;
Miyashita, Y ;
Kasai, H .
NEURON, 1999, 24 (04) :989-1002
[25]   Domains of high Ca2+ beneath the plasma membrane of living A7r5 cells [J].
Marsault, R ;
Murgia, M ;
Pozzan, T ;
Rizzuto, R .
EMBO JOURNAL, 1997, 16 (07) :1575-1581
[26]  
Mason W.T., 1999, FLUORESCENT LUMINESC
[27]  
Mattheakis LC, 2000, DRUG DISCOV TODAY, P15
[28]   Dynamic and quantitative Ca2+ measurements using improved cameleons [J].
Miyawaki, A ;
Griesbeck, O ;
Heim, R ;
Tsien, RY .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (05) :2135-2140
[29]   Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin [J].
Miyawaki, A ;
Llopis, J ;
Heim, R ;
McCaffery, JM ;
Adams, JA ;
Ikura, M ;
Tsien, RY .
NATURE, 1997, 388 (6645) :882-887
[30]   MONITORING DYNAMIC CHANGES IN FREE CA2+ CONCENTRATION IN THE ENDOPLASMIC-RETICULUM OF INTACT-CELLS [J].
MONTERO, M ;
BRINI, M ;
MARSAULT, R ;
ALVAREZ, J ;
SITIA, R ;
POZZAN, T ;
RIZZUTO, R .
EMBO JOURNAL, 1995, 14 (22) :5467-5475