Single molecule analysis of DNA immobilized on microspheres

被引：36

作者：

Osborne, MA ^{[1
]}

Furey, WS ^{[1
]}

Klenerman, D ^{[1
]}

Balasubramanian, S ^{[1
]}

机构：

[1] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England

来源：

ANALYTICAL CHEMISTRY | 2000年 / 72卷 / 15期

关键词：

D O I：

10.1021/ac000129r

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The formation and analysis of single molecules of fluorescently labeled DNA immobilized on polystyrene microspheres is described. Analysis by confocal fluorescence microscopy revealed single-step photobleaching, characteristic of a single fluorophore. Microspheres provide a means of locating single molecules by bright-field microscopy, prior to single-molecule detection. This allows the interrogation of single molecules without suffering the limitations of premature photobleaching. Statistical analysis of fluorescence intensities for >100 microspheres suggests attachment of DNA to micropsheres to be consistent with Poisson statistics.

引用

页码：3678 / 3681

页数：4

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