Characterization of gyrA mutations associated with fluoroquinolone resistance in Campylobacter coli by DNA sequence analysis and MAMA PCR

被引：80

作者：

Zirnstein, G

Helsel, L

Li, Y

Swaminathan, B

Besser, J

机构：

[1] Ctr Dis Control & Prevent, Natl Ctr Infect Dis, US PHS, US Dept HHS, Atlanta, GA 30333 USA

[2] Minnesota Dept Hlth, Minneapolis, MN USA

来源：

FEMS MICROBIOLOGY LETTERS | 2000年 / 190卷 / 01期

关键词：

Campylobacter coli; ciprofloxacin resistance; mismatch polymerase chain reaction; gyrA mutation;

D O I：

10.1016/S0378-1097(00)00306-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Increasing numbers of fluoroquinolone-resistant Campylobacter ter coli isolates received at the Minnesota State Public Health Laboratory and at the Centers for Disease Control and Prevention have been a cause for concern. The gyrA quinolone resistance-determining regions of several fluoroquinolone-resistant isolates were sequenced to examine the mechanism of resistance. Ciprofloxacin-resistant C. coli isolates examined by DNA sequencing had a Thr-86 to Ile (ACT --> ATT) gyrA mutation, leading to resistance to fluoroquinolone antibiotics. A mismatch amplification mutation assay polymerase chain reaction protocol was developed to detect this gyrA mutation. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

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页码：1 / 7

页数：7

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